Author | Monteiro, Juliana Santos de Carvalho | |
Author | Oliveira, Susana Carla Pires Sampaio | |
Author | Santos, Gustavo Miranda Pires | |
Author | Sampaio, Fernando José Pires | |
Author | Zanin, Fátima Antônia Aparecida | |
Author | Pinheiro, Antônio Luiz Barbosa | |
Access date | 2015-05-27T13:58:06Z | |
Available date | 2015-05-27T13:58:06Z | |
Document date | 2014 | |
Citation | MONTEIRO, J. S. C. et al. Effectiveness of antimicrobial photodynamic therapy on staphylococcus aureus using phenothiazinium dye with red laser. Proceedings of SPIE, v. 9309, p. 93090X-1, 2014. | pt_BR |
ISSN | 0277-786X | |
URI | https://www.arca.fiocruz.br/handle/icict/10570 | |
Language | eng | pt_BR |
Publisher | Society of Photo-optical Instrumentation Engineers (SPIE) | pt_BR |
Rights | open access | pt_BR |
Title | Effectiveness of Antimicrobial Photodynamic Therapy on Staphylococcus aureus using Phenothiazinium Dye with Red Laser | pt_BR |
Type | Article | pt_BR |
DOI | 10.1117/12.2079170 | |
Abstract | The aim of this study was to evaluate in vitro the bactericidal effect of Antimicrobial Photodynamic Therapy - AmPDT
using a phenothiazinium compound (toluidine blue O and methylene blue, 12.5 μg/mL) on Staphylococcus aureus
(ATCC 23529) irradiated or not with the red laser (λ 660 nm, 12J/cm2). All tests were performed in triplicate and
samples distributed into the following groups: Negative control, Laser, Photosensitizer, and AmPDT. Bactericidal effect
of the Antimicrobial Photodynamic Therapy was assessed by counting of colony-forming units and analyzed statistically
(ANOVA, Tukey test, p<0.05). The results showed, comparing the Laser group with Negative control, a statistically
significant increase of counting on the Laser group (p = 0.003). The use of the photosensitizer alone reduced the mean
number of CFU (64.8%) and its association with the Laser light resulted in 84.2% of inhibition. The results are indicative
that the use of Antimicrobial Photodynamic Therapy presented in vitro bactericidal effect on Staphylococcus aureus. | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil / Social Changes. Asthma and Allergy in Latin America. SCAALA. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Center of Biophotonic. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil | pt_BR |
Subject | Photodynamic antimicrobial chemotherapy | pt_BR |
Subject | Staphylococcus aureus | pt_BR |
Subject | Lasers | pt_BR |