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EVALUATION OF GAMMA INTERFERON IMMUNE RESPONSE ELICITED BY THE NEWLY CONSTRUCTED PSTS-1(285-374):CFP10 FUSION PROTEIN TO DETECT MYCOBACTERIUM TUBERCULOSIS INFECTION
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
The PstS1 antigen is highly immunogenic, principally when combined with CFP10 during both latent and active TB infection. In
the present study, a selected pstS1 gene fragment was cloned, fused with CFP10, and expressed in Escherichia coli. The product
[PstS-1(285-374):CFP10] was compared to the recombinant fused RD1 (region of deletion 1) protein (ESAT-6:CFP10) in detecting
Mycobacterium tuberculosis infection in 108 recent contacts of pulmonary tuberculosis (TB) cases, considering a positive
tuberculin skin test (TST) to be the baseline. The release of gamma interferon (IFN- ) in 22-h whole-blood and 5-day lymphocyte
stimulation assays primed with each antigen was determined. All contacts were clinically followed for up to 1 year, and 87%
of the tuberculin skin test-positive (TSTpositive) patients accepted preventative treatment. Concerning the IFN- response to
PstS-1(285-374):CFP10 in the 22-h and 5-day assays, a slight increase in contact-TSTpositive detection was observed (23/54 and
26/54) compared to the level seen with the RD1 protein (18/54 and 24/54) whereas in the TSTnegative group, similarly lower numbers
(<5/48) of responders were achieved for both antigens, except for RD1 in the 5-day assay (8/48). By combining the IFN-
responders to both antigens in the 5-day assays, slightly higher increases in positivity were found in the TSTpositive (32/54) and
TSTnegative (10/48) groups. Two of 12 untreated TSTpositive contacts progressed to active TB and were concordantly positive in all
assays, except for one contact who lacked positivity in the RD1 5-day assay. We demonstrated for the first time that PstS-1(285-
374):CFP10 slightly increased contact positivity and detection of active disease progression, suggesting its potential application
as a TB infection marker.
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