Author | Silva, Virgínia Maria Góes da | |
Author | Araújo, Cintia Figueiredo de | |
Author | Navarro, Isabela Cunha | |
Author | Oliveira, Pablo Rafael Silveira | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Access date | 2015-07-08T18:32:08Z | |
Available date | 2015-07-08T18:32:08Z | |
Document date | 2015 | |
Citation | SILVA, V. M. G. et al. Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity. BMC Research Notes, v. 8, p. 197, 2015. | pt_BR |
ISSN | 1756-0500 | |
URI | https://www.arca.fiocruz.br/handle/icict/11176 | |
Language | eng | pt_BR |
Publisher | Springer Verlag | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Antibody response | pt_BR |
Subject in Portuguese | Disease progression | pt_BR |
Title | Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13104-015-1158-0 | |
Abstract | BACKGROUND: Both Leishmania braziliensis and Leishmania amazonensis induce cutaneous disease when injected in the skin of BALB/c mice. However, L. amazonensis may also visceralize in that strain of mice, infecting mainly the liver and spleen. In addition, whereas BALB/c mice die with a progressive cutaneous disease when infected by L. amazonensis, the infection by L. braziliensis is spontaneously cured. In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract. METHODS: In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique. RESULTS: IgG1 antibodies recognizing an antigen with apparent molecular weight of 116 kDa were specifically detected in BALB/c mice that had been turned susceptible to L. braziliensis infection by injections of LaE. CONCLUSION: A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Estadual do Sudoeste da Bahia. Departamento de Ciências Biológicas. Jequié, BA, Brasil, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |