Author | Dorneles, Elaine Maria Seles | |
Author | Carvalho, Andréa Teixeira | |
Author | Araújo, Márcio Sobreira Silva | |
Author | Lima, Graciela Kunrath | |
Author | Martins Filho, Olindo Assis | |
Author | Sriranganathan, Nammalwar | |
Author | Lage, Andrey Pereira | |
Access date | 2015-07-13T14:22:05Z | |
Available date | 2015-07-13T14:22:05Z | |
Document date | 2014 | |
Citation | DORNELES, Elaine Maria Seles et al. T lymphocytes subsets and cytokine pattern induced by vaccination against bovine brucellosis employing S19 calfhood vaccination and adult RB51 revaccination. Vaccine, vol. 32, n. 46, p. 6034-8, 2014 | pt_BR |
ISSN | 0264-410X | |
URI | https://www.arca.fiocruz.br/handle/icict/11239 | |
Language | eng | pt_BR |
Publisher | Elsevier Science | pt_BR |
Rights | restricted access | pt_BR |
Title | T lymphocytes subsets and cytokine pattern induced by vaccination against bovine brucellosis employing S19 calfhood vaccination and adult RB51 revaccination | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.vaccine.2014.08.060 | |
Abstract | The aims of this study were to address the protective immune response induced by S19 vaccination (n=10) and RB51 revaccination, in pregnant (n=9) and non-pregnant (n=10) S19 calfhood-vaccinated cattle as follows: evaluate the in vitro CD4(+) and CD8(+) T-lymphocytes specific proliferation, and in vitro expression of IFN-γ by CD4(+) and CD8(+) T-cells and IL-4 by CD4(+), CD8(+) and CD21(+) lymphocytes subset. Upon in vitro stimulation with γ-irradiated Brucella abortus 2308, blood mononuclear cells from S19 vaccinated and RB51 revaccinated cows exhibited significantly higher proliferation of CD4(+) and CD8(+) T-lymphocytes and CD4(+)IFN-γ(+) T-cells compared to non-vaccinated animals. RB51 revaccination, regardless of the pregnancy status, did not enhance the proliferation of CD4(+) or CD8(+) T-cells nor IFN-γ or IL-4 production. Data from the present study suggest that cattle's cellular immune response induced after brucellosis vaccination and revaccination is due to CD4(+) and CD8(+) T-lymphocytes, being CD4(+) T-cells the main source of IFN-γ. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundacão Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoracão. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundacão Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoracão. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundacão Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoracão. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Virginia Polytechnic Institute and State University.Regional College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology. Center for Molecular Medicine and Infectious Diseases. Blacksburg, VA, USA | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Brucellosis | pt_BR |
Subject | Calfhood vaccination | pt_BR |
Subject | Cellular immunity | pt_BR |
Subject | RB51 | pt_BR |
Subject | Revaccination of pregnant cattle | pt_BR |
Subject | S19 | pt_BR |