Author | Fernandes, Ana Paula | |
Author | Coelho, Eduardo Antonio Ferraz | |
Author | Coelho, George Luiz Lins Machado | |
Author | Grimaldi Junior, Gabriel | |
Author | Gazzinelli, Ricardo Tostes | |
Access date | 2015-07-29T13:13:52Z | |
Available date | 2015-07-29T13:13:52Z | |
Document date | 2012 | |
Citation | FERNANDES, Ana Paula et al. Making an anti-amastigote vaccine for visceral leishmaniasis: rational, update and perspectives. Current Opinion in Microbiology, Vol. 15, n. 4, p. 476–485, 2012. | pt_BR |
ISSN | 1369-5274 | |
URI | https://www.arca.fiocruz.br/handle/icict/11416 | |
Language | eng | pt_BR |
Publisher | Elsevier Ltd. | pt_BR |
Rights | restricted access | pt_BR |
Title | Making an anti-amastigote vaccine for visceral leishmaniasis: rational, update and perspectives | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.mib.2012.05.002 | |
Abstract | Visceral leishmaniasis is a major health problem in Latina America, as well as the Mediterranean region of Europe and Asia. We aimed to develop a vaccine against visceral leishmaniasis targeting the intracellular amastigotes, which is the parasite stage that persists throughout infections with Leishmania parasites. With this in mind, we identified an amastigote specific antigen (A2) that contains an immunogenic epitope for CD4+ T helper (Th) cells and multiple repetitive units encoding CD8+ cytotoxic T lymphocyte (CTL) epitopes. Vaccine formulations containing the recombinant A2 associated with saponin, alum and IL-12 or expressed by attenuated adenovirus were shown to be protective in mice, dogs and nonhuman-primates. We are currently identifying novel amastigote specific immunogenic proteins that could be aggregated to A2 to further improve the level of vaccineinduced cell-mediated immunity and protection against visceral leishmaniasis | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Colégio Técnico. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Ouro Preto. Departamento de Ciências Médicas. Ouro Preto, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/ University of Massachusetts Medical School. Worcester, MA, USA | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | Vaccine | pt_BR |