Author | Cassemiro, Klécia Marília S. de Melo | |
Author | Burlandy, Fernanda M. | |
Author | Barbosa, Mikaela R. F. | |
Author | Chen, Qi | |
Author | Jorba, Jaume | |
Author | Hachich, Elayse M. | |
Author | Sato, Maria I. Z. | |
Author | Burns, Cara C. | |
Author | Silva, Edson E. da | |
Access date | 2016-06-19T19:56:47Z | pt_BR |
Access date | 2016-07-08T12:21:58Z | |
Available date | 2016-06-19T19:56:47Z | pt_BR |
Available date | 2016-07-08T12:21:58Z | |
Document date | 2016 | pt_BR |
Citation | CASSEMIRO, Klécia Marília S. de Melo;Molecular and Phenotypic Characterization of a Highly Evolved Type 2 Vaccine-Derived Poliovirus Isolated from Seawater in Brazil, 2014. Plos One, v.11, n.3, e0152251, 18p, Mar. 2016 | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14514 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Molecular and Phenotypic Characterization of a Highly Evolved Type 2 Vaccine-Derived Poliovirus Isolated from Seawater in Brazil, 2014 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0152251 | pt_BR |
Abstract | A type 2 vaccine-derived poliovirus (VDPV), differing from the Sabin 2 strain at 8.6% (78/
903) of VP1 nucleotide positions, was isolated from seawater collected from a seaport in
São Paulo State, Brazil. The P1/capsid region is related to the Sabin 2 strain, but
sequences within the 5'-untranslated region and downstream of the P1 region were derived
from recombination with other members of Human Enterovirus Species C (HEV-C). The two
known attenuating mutations had reverted to wild-type (A481G in the 5'-UTR and Ile143Thr
in VP1). The VDPV isolate had lost the temperature sensitive phenotype and had accumulated
amino acid substitutions in neutralizing antigenic (NAg) sites 3a and 3b. The date of
the initiating OPV dose, estimated from the number of synonymous substitutions in the capsid
region, was approximately 8.5 years before seawater sampling, a finding consistent with
a long time of virus replication and possible transmission among several individuals.
Although no closely related type 2 VDPVs were detected in Brazil or elsewhere, this VDPV
was found in an area with a mobile population, where conditions may favor both viral infection
and spread. Environmental surveillance serves as an important tool for sensitive and
early detection of circulating poliovirus in the final stages of global polio eradication. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Companhia Ambiental Do Estado De São Paulo. Departamento de Análises Ambientais. São Pulo, SP, Brasil. | pt_BR |
Affilliation | Centers for Disease Control and Prevention (CDC). Division of Viral Diseases,. Atlanta, GA, USA. | pt_BR |
Affilliation | Companhia Ambiental Do Estado De São Paulo. Departamento de Análises Ambientais. São Pulo, SP, Brasil | pt_BR |
Affilliation | Companhia Ambiental Do Estado De São Paulo. Departamento de Análises Ambientais. São Pulo, SP, Brasil. | pt_BR |
Affilliation | Centers for Disease Control and Prevention (CDC). Division of Viral Diseases,. Atlanta, GA, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil | pt_BR |
Subject | Molecular and phenotypic characterization | pt_BR |
Subject | Type 2 Vaccine-Derived Poliovirus | pt_BR |
Subject | Seawater | pt_BR |
Subject | São Sebastião,SP | pt_BR |