Author | Barreto-Vieira, Debora Ferreira | |
Author | Barth, Ortrud Monika | |
Author | Silva, Marcos Alexandre Nunes da | |
Author | Santos, Carolina Cardoso | |
Author | Santos, Aline da Silva | |
Author | F. Filho, Joaquim Batista | |
Author | Filippis, Ana Maria Bispo de | |
Access date | 2016-06-21T15:50:53Z | pt_BR |
Access date | 2016-07-07T11:41:08Z | |
Available date | 2016-06-21T15:50:53Z | pt_BR |
Available date | 2016-07-07T11:41:08Z | |
Document date | 2016 | pt_BR |
Citation | BARRETO-VIEIRA, Debora; et al. Ultrastructure of Zika virus particles in cell cultures. Mem Inst Oswaldo Cruz, Mar. 2016. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14604 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Zika virus | pt_BR |
Subject in Portuguese | Técnicas de Cultura de Células | pt_BR |
Title | Ultrastructure of Zika virus particles in cell cultures | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-02760160104 | pt_BR |
Abstract | Since 2015 Zika virus (ZIKV) infected thousands of Brazilian people and spread to
other American countries. Beside real time reverse transcriptase polimerase chain
reaction (RT-PCR) diagnosis detecting the viral genome, virus isolation in cell lines was
useful also and to study the viral particle structure and location inside the cells. This
tool was achieved using transmission electron microscopy (TEM) of ZIKV infected cell
lines. Human blood, obtained from a Brazilian patient during the first days of
symptoms, was isolated in C6/36 mosquito cells and inoculated in sequence in Vero
cells, fixed after six days, embedded in polymers, and ultrathin cut. This flavivirus, like
dengue viruses, showed numerous virus particles after replication occurring inside
vesicles of the endoplasmic reticulum confirming the susceptibility of the Vero cell line
to ZIKV. TEM is the unique technique disposable to make the virus really visible. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Laboratório Central de Saúde Pública do Espírito Santo/Virologia. ES, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Zika virus | pt_BR |
Subject | Virus particles | pt_BR |
Subject | Cell culture | pt_BR |
Subject | Transmission electron microcopy | pt_BR |
e-ISSN | 1678-8060 | pt_BR |