Author | Giarola, Naira Lígia Lima | |
Author | Amaral, Elmo Eduardo de Almeida | |
Author | Collopy Júnior, Itallo | |
Author | Souza, André Luiz Fonseca de | |
Author | Majerowicz, David | |
Author | Paes, Lisvane Silva | |
Author | Gondim, Katia C. | |
Author | Fernandes, José Roberto Meyer | |
Access date | 2016-08-25T12:10:32Z | |
Available date | 2016-08-25T12:10:32Z | |
Document date | 2013 | |
Citation | GIAROLA, Naíra Lígia Lima; et al. Trypanosoma cruzi: Effects of heat shock on ecto-ATPase activity. Experimental Parasitology, v.133, n.4, p.434-441, Apr. 2013. | pt_BR |
ISSN | 0014-4894 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15386 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Choque térmico | pt_BR |
Title | Trypanosoma cruzi: effects of heat shock on ecto-ATPase activity | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2012.12.014 | |
Abstract | In this work, we demonstrate that Trypanosoma cruzi Y strain epimastigotes exhibit Mg2+-dependent ecto-ATPase activity that is stimulated by heat shock. When the epimastigotes were incubated at 37°C for 2h, the ecto-ATPase activity of the cells was 43.95±0.97 nmol Pi/h×10(7) cells, whereas the ecto-ATPase activity of cells that were not exposed to heat shock stress was 16.97±0.30 nmol Pi/h×10(7) cells. The ecto-ATPase activities of cells, that were exposed or not exposed to heat shock stress had approximately the same Km values (2.25±0.26 mM ATP and 1.55±0.23 mM ATP, respectively) and different Vmax values. The heat-shocked cells had higher Vmax values (54.38±3.07 nmol Pi/h×10(7) cells) than the cells that were not exposed to heat shock (19.38±1.76 nmol Pi/h×10(7) cells). We also observed that the ecto-phosphatase and ecto-5'nucleotidase activities of cells that had been incubated at 28°C or 37°C were the same. Interestingly, cycloheximide, an inhibitor of protein synthesis, suppressed the heat shock effect of ecto-ATPase activity on T. cruzi. The Mg2+-dependent ecto-ATPase activity from the Y strain (high virulence) was approximately 2-fold higher than that of Dm28c (a clone with low virulence). In addition, these two strains presented different responses to heat shock with regard to their ecto-ATPase activities; Y strain epimastigotes had a stimulation of 2.52-fold while the Dm28c strain had a 1.71-fold stimulation. In this context, the virulent trypomastigote form of T. cruzi, Dm28c, had an ecto-ATPase activity that was more than 7-fold higher (66.67±5.98 nmol Pi/h×10(7) cells) than that of the insect epimastigote forms (8.91±0.76 nmol Pi/h×10(7) cells). This difference increased to approximately 10-fold when both forms were subjected to heat shock stress (181.14±16.48 nmol Pi/h×10(7) cells for trypomastigotes and 16.71±1.17 nmol Pi/h×10(7) cells for epimastigotes at 37°C). The ecto-ATPase activity of a plasma membrane-enriched fraction obtained from T. cruzi epimastigotes was not increased by heat treatment, which suggested that cytoplasmic components had an influence on enzyme activation by heat shock stress. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro – UFRJ. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Federal do Rio de Janeiro – IFRJ. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual da Zona Oeste – UEZO. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro – UFRJ. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Brasil. | pt_BR |
Affilliation | Universidade de São Paulo - USP. Departamento de Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro – UFRJ. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro – UFRJ. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Ecto-ATPase | pt_BR |
Subject | Heat shock | pt_BR |
Embargo date | 2030-01-01 | |