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Autor | Guimarães, Monick L. | |
Autor | Velarde-Dunois, Ketty G. | |
Autor | Segurondo, David | |
Autor | Morgado, Mariza G. | |
Fecha de acceso | 2016-09-15T15:36:31Z | |
Fecha de disponibilización | 2016-09-15T15:36:31Z | |
Fecha de publicación | 2012 | |
Referencia | GUIMARÃES, Monick L. et al. The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains. Virology Journal, v.9:19, 7p, 2012. | pt_BR |
ISSN | 1743-422X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15815 | |
Idioma | eng | pt_BR |
Editor | BioMed Central | pt_BR |
Derechos de autor | open access | pt_BR |
Palabras clave en Portugués | HIV-1 | pt_BR |
Palabras clave en Portugués | Bolívia | pt_BR |
Palabras clave en Portugués | Subtipos | pt_BR |
Título | The HIV-1 epidemic in Bolivia is dominated by subtype B and CRF12_BF “family” strains | pt_BR |
Tipo del documento | Article | pt_BR |
Resumen en Inglés | Background: Molecular epidemiological studies of HIV-1 in South America have revealed the occurrence of subtypes B, F1 and BF1 recombinants. Even so, little information concerning the HIV-1 molecular epidemiology in Bolivia is available. In this study we performed phylogenetic analyses from samples collected in Bolivia at two different points in time over a 10 year span. We analyzed these samples to estimate the trends in the HIV subtype and recombinant forms over time. Materials and methods: Fifty one HIV-1 positive samples were collected in Bolivia over two distinct periods (1996 and 2005). These samples were genetically characterized based on partial pol protease/reverse transcriptase (pr/rt) and env regions. Alignment and neighbor-joining (NJ) phylogenetic analyses were established from partial env (n = 37) and all pol sequences using Mega 4. The remaining 14 env sequences from 1996 were previously characterized based on HMA-env (Heteroduplex mobility assay). The Simplot v.3.5.1 program was used to verify intragenic recombination, and SplitsTree 4.0 was employed to confirm the phylogenetic relationship of the BF1 recombinant samples. Results: Phylogenetic analysis of both env and pol regions confirmed the predominance of “pure” subtype B (72.5%) samples circulating in Bolivia and revealed a high prevalence of BF1 genotypes (27.5%). Eleven out of 14 BF1 recombinants displayed a mosaic structure identical or similar to that described for the CRF12_BF variant, one sample was classified as CRF17_BF, and two others were F1pol/Benv. No “pure” HIV-1 subtype F1 or B” variant of subtype B was detected in the present study. Of note, samples characterized as CRF12_BF-related were depicted only in 2005. Conclusion: HIV-1 genetic diversity in Bolivia is mostly driven by subtype B followed by BF1 recombinant strains from the CRF12_BF “family”. No significant temporal changes were detected between the mid-1990s and the mid- 2000s for subtype B (76.2% vs 70.0%) or BF1 recombinant (23.8% vs 30.0%) samples from Bolivia. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Policía Nacional. La Paz, Bolivia. | pt_BR |
Afiliación | Ministerio de la Salud. Programa Departamental de ITS, VIH/SIDA. La Paz, Bolivia. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Palavras clave en Inglês | HIV-1 | pt_BR |
Palavras clave en Inglês | Subtypes | pt_BR |
Palavras clave en Inglês | Bolivia | pt_BR |
Palavras clave en Inglês | CRF | pt_BR |
e-ISSN | 1743-422X |
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