Author | Souza, Fernando Almeida | |
Author | Souza, Celeste da Silva Freitas de | |
Author | Taniwaki, Noemi Nosomi | |
Author | Silva, João José Mendes | |
Author | Oliveira, Renata Mondêgo de | |
Author | Silva, Ana Lúcia Abreu | |
Author | Calabrese, Kátia da Silva | |
Access date | 2016-12-20T11:46:08Z | |
Available date | 2016-12-20T11:46:08Z | |
Document date | 2016 | |
Citation | SOUZA, Fernando Almeida et al. Morinda citrifolia Linn. fruit (Noni) juice induces an increase in NO production and death of Leishmania amazonenis amastigotes in peritoneal macrophages form BALB/c. Nitric Oxide: Biology and Chemistry, v. 58, p. 51-58, June 2016. | pt_BR |
ISSN | 1089-8603 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16558 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Morinda | pt_BR |
Subject in Portuguese | Leishmaniose | pt_BR |
Subject in Portuguese | Óxido Nítrico | pt_BR |
Subject in Portuguese | Microscopia eletrônica | pt_BR |
Subject in Portuguese | Macrófagos | pt_BR |
Title | Morinda citrifolia Linn. fruit (Noni) juice induces an increase in NO production and death of Leishmania amazonensis amastigotes in peritoneal macrophages from BALB/c | pt_BR |
Type | Article | pt_BR |
Abstract | Leishmaniasis is a complex disease that is considered a serious public health problem. Due to the absence of an effective vaccine and debilitating chemotherapy better therapies are urgently needed. This situation has stimulated the search for alternative treatments such as the use of herbal medicines. Several studies conducted with Morinda citrifolia Linn. have shown various biological activities such as antitumor, immunomodulation and antileishmanial activity, however its mechanisms of action are still unknown. This study aimed to analyze the activity of M. citrifolia fruit juice against Leishmania amazonensis and its action on peritoneal macrophages from BALB/c infected with L. amazonensis. Activity against the promastigote forms showed IC50 at 275.3 μg/mL. Transmission electron microscopy was used to evaluate the ultrastructural alterations in the promastigotes treated with the juice and the results showed cytoplasmic vacuolization, lipid inclusion and increased activity of exocytosis. The juice treatment presented an IC50 at 208.4 μg/mL against intracellular amastigotes and led to an increased nitrite production in infected and non-infected macrophages. When macrophages were pre-treated with iNOS inhibitors, aminoguanidine or 1400W, the intracellular amastigotes increased, demonstrating the important role of NO production in M. citrifolia fruit activity. In conclusion, our results reveal that treatment with M. citrifolia fruit juice can increase NO production in peritoneal macrophages and this ability has an important role in the killing of L. amazonensis intracellular amastigotes. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. Departamento de Patologia. São Luís, MA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Adolfo Lutz. Unidade de Microscopia Eletrônica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Maranhão. Departamento de Engenharia Agrícola. São Luís, MA, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luís, MA, Brasil. | pt_BR |
Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luís, MA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Morinda citrifolia | pt_BR |
Subject | Noni | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Nitric oxide | pt_BR |
Subject | Electron microscopy | pt_BR |
Subject | Macrophages | pt_BR |
Subject | Amino guanidine | pt_BR |
e-ISSN | 10.1016/j.niox.2016.06.004 | |
Embargo date | 2030-12-31 | |