Author | Santos, Silvana Ornelas | |
Author | Fontes, Jonathan Luís Magalhães | |
Author | Laranjeira, Daniela Farias | |
Author | Vassallo, José | |
Author | Melo, Stella Maria Barrouin | |
Author | dosSantos, Washington Luis Conrado | |
Access date | 2016-12-26T19:23:47Z | |
Available date | 2016-12-26T19:23:47Z | |
Document date | 2016 | |
Citation | SANTOS, S. O. et al. A minimally invasive approach to spleen histopathology in dogs: A new method for follow-up studies of spleen changes in the course of Leishmania infantum infection. Comparative Immunology, Microbiology and Infectious Diseases, v. 48, p. 87–92, 2016. | pt_BR |
ISSN | 0147-9571 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/16564 | |
Sponsorship | AUXPE. CAPES. FAPESB 2072/2013/PROCESSO#23038.006718/2013-19 and Fundação Oswaldo Cruz | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania infantum | pt_BR |
Subject in Portuguese | Cão | pt_BR |
Subject in Portuguese | Leishmaniose visceral canina | pt_BR |
Subject in Portuguese | Baço | pt_BR |
Subject in Portuguese | Bloco celular | pt_BR |
Subject in Portuguese | Biopsia com agulha fina | pt_BR |
Subject in Portuguese | Histopatologia | pt_BR |
Title | A minimally invasive approach to spleen histopathology in dogs: A new method for follow-up studies of spleen changes in the course of Leishmania infantum infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.cimid.2016.08.007 | |
Abstract | Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher's test, P<0.02) and a higher density of plasma cells (score 3, Fisher's test, P<0.02) than uninfected dogs (5/7 exhibiting lymphoid follicles and a plasma cell score of 1). The dogs with Leishmania infection also presented with granulomas (8/13) and infected macrophages (5/13). These differences in the histological presentations of spleen tissue from infected and uninfected dogs corresponded to changes observed in conventional histology. Hence, the cell-block technique described here may be used in the follow-up care and study of dogs with ZVL and other diseases in both clinical practice and research. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departmento de Anatomia, Patologia and Clínicas. Salvador, BA, Brasil | pt_BR |
Affilliation | UNICAMP. Faculdade de Ciências Médicas. Campinas, SP, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departmento de Anatomia, Patologia and Clínicas. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Dog | pt_BR |
Subject | Canine visceral leishmaniasis | pt_BR |
Subject | Spleen | pt_BR |
Subject | Cell block | pt_BR |
Subject | Fine-needle biopsy | pt_BR |
Subject | Minimally invasive histopathologya | pt_BR |