Author | Fouts, Derrick E | |
Author | Matthias, Michael A | |
Author | Adhikarla, Haritha | |
Author | Adler, Ben | |
Author | Santos, Luciane Amorim | |
Author | Berg, Douglas E | |
Author | Bulach, Dieter | |
Author | Buschiazzo, Alejandro | |
Author | Chang, Yung-Fu | |
Author | Galloway, Renee L | |
Author | Haake, David A | |
Author | Haft, Daniel H | |
Author | Hartskeerl, Rudy | |
Author | Ko, Albert Icksang | |
Author | Levett, Paul N | |
Author | Matsunaga, James | |
Author | Mechaly, Ariel E | |
Author | Monk, Jonathan M | |
Author | Nascimento, Ana Lúcia Tablet Oller do | |
Author | Nelson, Karen E | |
Author | Palsson, Bernhard | |
Author | Peacock, Sharon J | |
Author | Picardeau, Mathieu | |
Author | Ricaldi, Jessica N | |
Author | Thaipandungpanit, Janjira | |
Author | Wunder Junior, Elsio Augusto | |
Author | Yang, X Frank | |
Author | Zhang, Jun-Jie | |
Author | Vinetz, Joseph M | |
Access date | 2017-02-23T16:47:12Z | |
Available date | 2017-02-23T16:47:12Z | |
Document date | 2016 | |
Citation | FOUTS, D. E. et al. What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus Leptospira. Plos Neglected Tropical Diseases, v. 10, n. 2, e0004403, 2016. | pt_BR |
ISSN | 1935-2727 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/17896 | |
Sponsorship | National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leptospira | pt_BR |
Subject in Portuguese | Leptospirose | pt_BR |
Subject in Portuguese | Evolução molecular | pt_BR |
Subject in Portuguese | Filogenia | pt_BR |
Subject in Portuguese | Animais | pt_BR |
Subject in Portuguese | Virulência | pt_BR |
Subject in Portuguese | Genoma bacteriano | pt_BR |
Title | What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus Leptospira | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0004403 | |
Abstract | Leptospirosis, caused by spirochetes of the genus Leptospira, is a globally widespread, neglected and emerging zoonotic disease. While whole genome analysis of individual pathogenic, intermediately pathogenic and saprophytic Leptospira species has been reported, comprehensive cross-species genomic comparison of all known species of infectious and non-infectious Leptospira, with the goal of identifying genes related to pathogenesis and mammalian host adaptation, remains a key gap in the field. Infectious Leptospira, comprised of pathogenic and intermediately pathogenic Leptospira, evolutionarily diverged from non-infectious, saprophytic Leptospira, as demonstrated by the following computational biology analyses: 1) the definitive taxonomy and evolutionary relatedness among all known Leptospira species; 2) genomically-predicted metabolic reconstructions that indicate novel adaptation of infectious Leptospira to mammals, including sialic acid biosynthesis, pathogen-specific porphyrin metabolism and the first-time demonstration of cobalamin (B12) autotrophy as a bacterial virulence factor; 3) CRISPR/Cas systems demonstrated only to be present in pathogenic Leptospira, suggesting a potential mechanism for this clade's refractoriness to gene targeting; 4) finding Leptospira pathogen-specific specialized protein secretion systems; 5) novel virulence-related genes/gene families such as the Virulence Modifying (VM) (PF07598 paralogs) proteins and pathogen-specific adhesins; 6) discovery of novel, pathogen-specific protein modification and secretion mechanisms including unique lipoprotein signal peptide motifs, Sec-independent twin arginine protein secretion motifs, and the absence of certain canonical signal recognition particle proteins from all Leptospira; and 7) and demonstration of infectious Leptospira-specific signal-responsive gene expression, motility and chemotaxis systems. By identifying large scale changes in infectious (pathogenic and intermediately pathogenic) vs. non-infectious Leptospira, this work provides new insights into the evolution of a genus of bacterial pathogens. This work will be a comprehensive roadmap for understanding leptospirosis pathogenesis. More generally, it provides new insights into mechanisms by which bacterial pathogens adapt to mammalian hosts. | pt_BR |
Affilliation | J. Craig Venter Institute. Rockville, Maryland, USA | pt_BR |
Affilliation | University of California San Diego School of Medicine. Division of Infectious Diseases. Department of Medicine. La Jolla, California, USA | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USA | pt_BR |
Affilliation | Monash University. Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics. Department of Microbiology. Clayton, Australia | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of California San Diego School of Medicine. Division of Infectious Diseases. Department of Medicine. La Jolla, California, USA | pt_BR |
Affilliation | Monash University. Victorian Bioinformatics Consortium. Clayton, Victoria, Australia | pt_BR |
Affilliation | Institut Pasteur de Montevideo. Laboratory of Molecular and Structural Microbiology. Montevideo, Uruguay / Institut Pasteur. Department of Structural Biology and Chemistry. Paris, France | pt_BR |
Affilliation | Cornell University. College of Veterinary Medicine. Department of Population Medicine & Diagnostic Sciences. Ithaca, New York, USA | pt_BR |
Affilliation | Centers for Disease Control and Prevention (DHHS, CDC, OID, NCEZID, DHCPP, BSPB). Atlanta, Georgia, USA | pt_BR |
Affilliation | VA Greater Los Angeles Healthcare System. Los Angeles California, USA / David Geffen School of Medicine at UCLA. Los Angeles, California, USA | pt_BR |
Affilliation | J. Craig Venter Institute. Rockville, Maryland, USA | pt_BR |
Affilliation | WHO/FAO/OIE and National Collaborating Centre for Reference and Research on Leptospirosis. Royal Tropical Institute (KIT). KIT Biomedical Research. Amsterdam, The Netherlands | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Government of Saskatchewan. Disease Control Laboratory Regina. Canada | pt_BR |
Affilliation | VA Greater Los Angeles Healthcare System. Los Angeles California, USA / David Geffen School of Medicine at UCLA. Los Angeles, California, USA | pt_BR |
Affilliation | Institut Pasteur de Montevideo. Laboratory of Molecular and Structural Microbiology. Montevideo, Uruguay | pt_BR |
Affilliation | University of California. Department of Bioengineering. San Diego, La Jolla, California, USA | pt_BR |
Affilliation | Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil / Instituto de Ciências Biomédicas USP. Programa Interunidades em Biotecnologia. São Paulo, SP, Brasil | pt_BR |
Affilliation | J. Craig Venter Institute. Rockville, Maryland, USA | pt_BR |
Affilliation | University of California. Department of Bioengineering. San Diego, La Jolla, California, USA | pt_BR |
Affilliation | University of Cambridge. Department of Medicine. Cambridge, United Kingdom | pt_BR |
Affilliation | Institut Pasteur. Biology of Spirochetes Unit. National Reference Centre and WHO Collaborating Center for Leptospirosis. Paris, France | pt_BR |
Affilliation | Universidd Peruana Cayetano Heredia. Facultad de Medicina Alberto Hurtado. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru | pt_BR |
Affilliation | Mahidol University. Faculty of Tropical Medicine. Bangkok, Thailand | pt_BR |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Indiana University School of Medicine. Department of Microbiology and Immunology. Indianapolis, Indiana, USA | pt_BR |
Affilliation | Indiana University School of Medicine. Department of Microbiology and Immunology. Indianapolis, Indiana, USA | pt_BR |
Affilliation | University of California San Diego School of Medicine. Division of Infectious Diseases. Department of Medicine. La Jolla, California, USA / Universidd Peruana Cayetano Heredia. Facultad de Medicina Alberto Hurtado. Instituto de Medicina Tropical Alexander von Humboldt. Lima, Peru / Universidad Peruana Cayetano Heredia. Instituto de Medicina “Alexander von Humboldt,”. Lima, Peru | pt_BR |
Subject | Leptospira | pt_BR |
Subject | Leptospirosis | pt_BR |
Subject | Evolution, molecular | pt_BR |
Subject | Phylogeny | pt_BR |
Subject | Animals | pt_BR |
Subject | Virulence | pt_BR |
Subject | Genome, bacterial | pt_BR |