Author | Oliveira, Felipe Pinheiro de | |
Author | Mendes, Roberta Hack | |
Author | Dobbler, Priscila Thiago | |
Author | Mai, Volker | |
Author | Pylro, Victor Satler | |
Author | Waugh, Sheldon G | |
Author | Vairo, Filippo | |
Author | Refosco, Lilia Farret | |
Author | Roesch, Luiz Fernando Würdig | |
Author | Schwartz, Ida Vanessa Doederlein | |
Access date | 2017-04-12T19:10:17Z | |
Available date | 2017-04-12T19:10:17Z | |
Document date | 2016 | |
Citation | OLIVEIRA, Felipe Pinheiro de et al. Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. PLoS ONE, v. 11, n. 6, e0157513, 2016 | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18434 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | fenilcetonuria | pt_BR |
Title | Phenylketonuria and Gut Microbiota: A Controlled Study Based on Next-Generation Sequencing. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.015751 | |
Abstract | Phenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™ platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear. | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório Basic Research and Advanced Investigations in Neurosciences. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, Brazil | pt_BR |
Affilliation | University of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of America | pt_BR |
Affilliation | Oswaldo Cruz Fundation. René Rachou Research Centre. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | University of Florida. Emerging Pathogens Institute. College of Public Health and Health Professions and College of Medicine. Department of Epidemiology. Gainesville, FL, United States of America | pt_BR |
Affilliation | Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre, RS, Brazil | pt_BR |
Affilliation | Universidade Federal do Pampa. Centro Interdisciplinar de Pesquisas em Biotecnologia–CIP-Biotec. São Gabriel, RS, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil/Hospital de Clínicas de Porto Alegre. Centro de Pesquisa Experimental. Laboratório de Pesquisas Básicas e Aplicadas em Neurociencias. Porto Alegre, RS, Brazil | pt_BR |
Subject | Phenylketonuria | pt_BR |
Subject | Microbiome | pt_BR |
Subject | Gut bactéria | pt_BR |
Subject | Diet | pt_BR |
Subject | Microbial ecology | pt_BR |
Subject | Clostridium | pt_BR |
Subject | Ribosomal RNA | pt_BR |
Subject | Protein metabolism | pt_BR |