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- IOC - Artigos de Periódicos [12507]
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MYCOBACTERIAL LAMININ-BINDING HISTONE-LIKE PROTEIN MEDIATES COLLAGEN-DEPENDENT CYTOADHERENCE
cytoadherence
collagen
histone-like protein
leprosy
tuberculosis
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.
Inserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.
Université Lille Nord de France. Lille, France.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.
sem afiliação
Inserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.
INRA, UR1282, Infectiologie et Santé Publique. Centre de Tours. Nouzilly, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.
Inserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.
Université Lille Nord de France. Lille, France.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.
sem afiliação
Inserm, U 1019. Lille, France / CNRS. UMR 8204 7CNRS. UMR 8525. Lille, France / Institut Pasteur de Lille. Centre for Infection and Immunity of Lille (CIIL). Lille, France / Université Lille Nord de France. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.
INRA, UR1282, Infectiologie et Santé Publique. Centre de Tours. Nouzilly, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Molecular. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
When grown in the presence of exogenous collagen I, Mycobacterium bovis BCG was shown to form clumps. Scanning electron microscopy examination of these clumps revealed the presence of collagen fibres cross-linking the bacilli. Since collagen is a major constituent of the eukaryotic extracellular matrices, we assayed BCG cytoadherence in the presence of exogenous collagen I. Collagen increased the interaction of the bacilli with A549 type II pneumocytes or U937 macrophages, suggesting that BCG is able to recruit collagen to facilitate its attachment to host cells. Using an affinity chromatography approach, we have isolated a BCG collagen-binding protein corresponding to the previously described mycobacterial laminin-binding histone-like protein (LBP/Hlp), a highly conserved protein associated with the mycobacterial cell wall. Moreover, Mycobacterium leprae LBP/Hlp, a wellcharacterized adhesin, was also able to bind collagen I. Finally, using recombinant fragments of M. leprae LBP/ Hlp, we mapped the collagen-binding activity within the C-terminal domain of the adhesin. Since this protein was already shown to be involved in the recognition of laminin and heparan sulphate-containing proteoglycans, the present observations reinforce the adhesive activities of LBP/Hlp, which can be therefore considered as a multifaceted mycobacterial adhesin, playing an important role in both leprosy and tuberculosis pathogenesis
Palavras-chave em inglês
Mycobacteriacytoadherence
collagen
histone-like protein
leprosy
tuberculosis
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