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https://www.arca.fiocruz.br/handle/icict/19087
EFFICACY OF THE PHOTODYNAMIC ANTIMICROBIAL THERAPY (PACT) WITH THE USE OF METHYLENE BLUE ASSOCIATED WITH THE λ660NM LASER IN LEISHMANIA (LEISHMANIA) AMAZONENSIS: IN VITRO STUDY
Autor(es)
Afiliação
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brazil
Resumo em Inglês
The present studied evaluated the in vitro effects of PDT on Leishmania (Leishmania) amazonensis promastigotes. For
this examination L. amazonensis promastigotes, stain Josefa, were used and maintained in Warren media supplement
with fetal bovine serum at 26°C for 96 hours. A viability curve was accomplished using different concentrations of
methylene blue photosensitizer associated to red laser light in order to obtain the most effective interaction to inhibit the
parasite’s growth. Two pre-irradiation periods, 5 and 30 minutes, were evaluated and the promastigotes were counted by
colorimetry. On fluorescence microscopy the autophagic processes and reactive oxygen species were detected.
Promastigotes treated with Photodynamic Therapy (PDT) by concentrations of 5 and 0,315ug/mL, presented cellular
proliferation inhibition when compared to the control. In the first condition, the cells had structural alterations such as
truncated cells, cells with two flagella, bleb formation and cells body deformation, while none of these modifications
could be visualized in the control group. When analyzed through fluorescence microscopy, the promastigotes treated
were positives for free radicals immediately after light application and also 1 hour after treatment presenting signs of
autophagia. PDT on L. (L.) amazonensis is effective causing alterations that can help elucidate the mechanisms of the
parasite’s death when treated with methilene blue associated to laser light. Therefore, new studies of PDT intra-parasite’s
proceedings are still being accomplished.
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