Author | Cuervo, Patricia | |
Author | Jesus, Jose Batista de | |
Author | Junqueira, Magno | |
Author | Lima, Leila Mendonça | |
Author | González, Luis Javier | |
Author | Betancourt, Lázaro | |
Author | Grimaldi Jr., Gabriel | |
Author | Domont, Gilberto Barbosa | |
Author | Fernandes, Octavio | |
Author | Cupolillo, Elisa | |
Access date | 2017-06-06T14:20:18Z | |
Available date | 2017-06-06T14:20:18Z | |
Document date | 2007 | |
Citation | CUERVO, Patricia; et al. Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry. Molecular & Biochemical Parasitology, v.154, n.1, p.6–21, July 2007. | pt_BR |
ISSN | 0166-6851 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/19318 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Leishmaniose | pt_BR |
Subject in Portuguese | Proteoma | pt_BR |
Subject in Portuguese | Espectrometria de Massas | pt_BR |
Subject in Portuguese | Mapa de referência 2D | pt_BR |
Title | Proteome analysis of Leishmania (Viannia) braziliensis by two-dimensional gel electrophoresis and mass spectrometry | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.molbiopara.2007.03.013 | |
Abstract | Leishmania (Viannia) braziliensis, a protozoan parasite widespread in the New World, is responsible for the infection of different mammal orders, including humans. This species is considered to be a major etiological agent of American cutaneous leishmaniasis. A proteomic study was carried out to identify proteins expressed by L. (V.) braziliensis. One hundred and one spots representing 75 protein entries were identified by MALDI-TOF-TOF. Isoelectric point values estimated by gel electrophoresis matched closely with predicted values, although some discrepancies existed suggesting that post-translational protein modifications may be common in L. braziliensis. Moreover, 20 hypothetical proteins were experimentally identified. Identified proteins were classified into 15 groups according to biological process. Among the proteins identified, approximately 40% have not been previously reported in a proteomic map of Leishmania. In addition, a number of potential virulence factors and drug targets were identified in this protein map, including some proteins associated with the metastatic phenotype. This study describes the first compilation of a proteomic reference map for L. braziliensis (pI 4-7, M(r) 10-130 kDa) and provides a very useful tool for comparative studies of strains isolated from patients presenting different clinical manifestations of leishmaniasis as well as a potential tool to identify markers for clinical diagnosis, therapeutics, and prognosis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento Medicina Tropical. Rio de Janeiro, RJ., Brasil / Centro Internacional de Entrenamiento e Investigaciones Médicas - CIDEIM. Cali, Colombia. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro / Rede Proteômica de Rio de Janeiro. Instituto de Química. Departamento de Bioqúímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Centro de Ingenieríıa Genética y Biotecnología. Departamento de Proteómica. Ciudad de la Habana, Cuba. | pt_BR |
Affilliation | Centro de Ingenieríıa Genética y Biotecnología. Departamento de Proteómica. Ciudad de la Habana, Cuba. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro / Rede Proteômica de Rio de Janeiro. Instituto de Química. Departamento de Bioqúímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento Medicina Tropical. Rio de Janeiro, RJ., Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Leishmania (V.) braziliensis | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Proteome | pt_BR |
Subject | Mass spectrometry | pt_BR |
Subject | Leishmania braziliensis 2D reference map | pt_BR |
e-ISSN | 0166-6851 | |
Embargo date | 2030-01-01 | |