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https://www.arca.fiocruz.br/handle/icict/20034
DISTRIBUTION AND ANALYSIS OF SURFACE CHARGE ON BRAIN ENDOTHELIUM IN VITRO AND IN SITU
Carga negativa
Glicosaminoglicanos
Sulfato de Heparan
Ácido siálico
Affilliation
Institute of Psychiatry. Department of Neuropathology. London, UK
Institute of Psychiatry. Department of Neuropathology. London, UK
Institute of Child Health. Molecular Immunology Unit. London, UK
Institute of Psychiatry. Department of Neuropathology. London, UK
Institute of Psychiatry. Department of Neuropathology. London, UK
Institute of Child Health. Molecular Immunology Unit. London, UK
Institute of Psychiatry. Department of Neuropathology. London, UK
Abstract
Vascular endothelial cells are associated with a
number of anionic molecules. These anions are important
in endothelial function, particularly in regulating permeability,
haemostasis and cellular traffic. To explore the nature
and distribution of anions on the brain endothelial
cell (BEC) surface, we have examined rat brain endothelium
in culture, and in situ. The anionic sites were probed
with cationic colloidal gold and cationised ferritin, and visualised
by light microscopy. Additionally we compared
the distribution of the anionic sites on BEC with that present
on other endothelial cell types in culture. The predominant
anion detected on BEC was heparan sulphate
(HS). This was distributed throughout the cell membrane,
but was most densely associated with intercellular junctions.
This pattern was distinct from the anionic locations
observed in endothelia from aorta and epididymal fat microvessels.
The distribution of anions was dependent on
the age of cultured cells, with only minimal levels of HS
seen at the periphery of younger cells. The nature and distribution
of negative charge was different in situ. Here,
sialic acid was the major surface anion, with only a small
contribution from HS. The significance of these findings
are discussed in relation to endothelial function in normal
tissue and in pathological conditions.
Keywords in Portuguese
EndotélioCarga negativa
Glicosaminoglicanos
Sulfato de Heparan
Ácido siálico
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