Author | Greenwood, J. | |
Author | Pryce, G. | |
Author | Devine, L. | |
Author | Male, D. K. | |
Author | Santos, Washington Luis Conrado dos | |
Author | Calder, V. L. | |
Author | Adamson, P. | |
Access date | 2017-07-11T13:03:00Z | |
Available date | 2017-07-11T13:03:00Z | |
Document date | 1996 | |
Citation | GREENWOOD, J. et al. SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology, v. 71, p. 51-63, 1996. | pt_BR |
ISSN | 0165-5728 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20037 | |
Sponsorship | dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Barreira hematoencefalica | pt_BR |
Subject in Portuguese | Barreira sangue-retina | pt_BR |
Subject in Portuguese | Endotélio | pt_BR |
Subject in Portuguese | Epitélio pigmentado da retina | pt_BR |
Title | SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics | pt_BR |
Type | Article | pt_BR |
Abstract | In the central nervous system the blood–brain and blood–retinal barriers (BBBand BRBrespectively)are instrumentalin maintaining
homeostasisof the neural parenchymaand controllingleucocytetraffic. These cellular barriers are formed primarilyby the vascular
endotheliumof the brain and retina althoughin the latter the pigmentedepithelial cells also form part of the barrier. From primary
culturesof rat brain endothelium,retinal endotheliumand retinal pigmentepitheiium(RPE) we have generatedtemperaturesensitive
SV40largeT immortalisedcell lines. Clonesof brain (GP8.3)and retinal(JG2.1)endotheliaand RPE (LD7.4)have been derivedfrom
parent lines that expressthe large T antigen at the permissivetemperature.The endothelialcell (EC) lines expressedP-glycoprotein,
GLUT-1,the transfernnreceptor,von Willebrandfactor and the RECA-1antigenand exhibitedhigh affinityuptakeof acetylatedLDL
and stainedpositivewith the lectin Griffoniasimplicifolia.The RPE cell line was positivefor cytokeratinsand for the rat RPE antigen
RET-PE2.All the cell lines expressedmajor histocompatibilitycomplex(MHC)class 1 and intercellularadhesionmolecule(ICAM)-1
constitutivelyand could be induced to expressMHC class II and vascular cell adhesion molecule (VCAM)-1following cytokine
activation.The EC also expressedplatelet endothelialcelI adhesionmolecule(PECAM)-I.Monolayer of these cells could supportthe
migrationof antigen-specificT cell lines.Thegenerationof immortalisedcell linesderivedfromthe rat BBBandBRBshouldproveto be
usefultools for the studyof these specialisedcellularbarriers. | pt_BR |
Affilliation | University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UK | pt_BR |
Affilliation | University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UK | pt_BR |
Affilliation | University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UK | pt_BR |
Affilliation | Institute of Psychiatry. Department of Neuropathology. London, UK | pt_BR |
Affilliation | Institute of Psychiatry. Department of Neuropathology. London, UK | pt_BR |
Affilliation | University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UK | pt_BR |
Affilliation | University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UK | pt_BR |
Subject | Blood–brain barrier | pt_BR |
Subject | Blood–retinal barrier | pt_BR |
Subject | Endothelium | pt_BR |
Subject | Immortalisation | pt_BR |
Subject | Retinal pigment epitheliums | pt_BR |