Mostrar el registro sencillo del ítem
Autor | Nascimento, Nathaly Alexandre do | |
Autor | Ferreira, Lígia Maria | |
Autor | Romão, Tatiany Patrícia | |
Autor | Correia, Darleide Maria da Conceição | |
Autor | Vasconcelos, Crhisllane Rafaele dos Santos | |
Autor | Rezende, Antônio Mauro | |
Autor | Costa, Samara Graciane | |
Autor | Genta, Fernando Ariel | |
Autor | Melo Neto, Osvaldo Pompílio | |
Autor | Silva Filha, Maria Helena Neves Lobo | |
Fecha de acceso | 2017-07-13T14:08:36Z | |
Fecha de disponibilización | 2017-07-13T14:08:36Z | |
Fecha de publicación | 2017 | |
Referencia | NASCIMENTO, Nathaly Alexandre do; et al. N-glycosylation influences the catalytic activity of mosquito a-glucosidases associated with susceptibility or refractoriness to Lysinibacillus sphaericus. Insect Biochemistry and Molecular Biology, v.81, p.62-71, 2017. | pt_BR |
ISSN | 0965-1748 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/20074 | |
Idioma | eng | pt_BR |
Editor | Elsevier | pt_BR |
Derechos de autor | restricted access | pt_BR |
Palabras clave en Portugués | Aedes aegypti | pt_BR |
Palabras clave en Portugués | Glucosidases | pt_BR |
Palabras clave en Portugués | N-Glicosilação | pt_BR |
Título | N-glycosylation influences the catalytic activity of mosquito α-glucosidases associated with susceptibility or refractoriness to Lysinibacillus sphaericus | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.1016/j.ibmb.2016.12.009 | |
Resumen en Inglés | Cqm1 and Aam1 are α-glucosidases (EC 3.2.1.20) expressed in Culex quinquefasciatus and Aedes aegypti larvae midgut, respectively. These orthologs share high sequence similarity but while Cqm1 acts as a receptor for the Binary (Bin) insecticidal toxin from Lysinibacillus sphaericus, Aam1 does not bind the toxin, rendering Ae. aegypti refractory to this bacterium. Aam1 is heavily glycosylated, contrasting to Cqm1, but little is known regarding how glycosylation impacts on its function. This study aimed to compare the N-glycosylation patterns and the catalytic activities of Aam1 and Cqm1. Mutant proteins were generated where predicted Aam1 N-glycosylation sites (N-PGS) were either inserted into Cqm1 or abrogated in Aam1. The mutants validated four N-PGS which were found to localize externally on the Aam1 structure. These Aam1 and Cqm1 mutants maintained their Bin binding properties, confirming that glycosylation has no role in this interaction. The α-glucosidase activity of both proteins was next investigated, with Aam1 having a remarkably higher catalytic efficiency, influenced by changes in glycosylation. Molecular dynamics showed that glycosylated and nonglycosylated Aam1 models displayed distinct patterns that could influence their catalytic activity. Differential N-glycosylation may then be associated with higher catalytic efficiency in Aam1, enhancing the functional diversity of related orthologs. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
Palavras clave en Inglês | Cqm1 | pt_BR |
Palavras clave en Inglês | Aam1 | pt_BR |
Palavras clave en Inglês | Culex quinquefasciatus | pt_BR |
Palavras clave en Inglês | Aedes aegypti | pt_BR |
Palavras clave en Inglês | N-glycosylation | pt_BR |
Palavras clave en Inglês | Glucosidases | pt_BR |
e-ISSN | 1879-0240 | |
Fecha de embargo | 2030-01-01 |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
IOC - Artigos de Periódicos [12500]