Por favor, use este identificador para citar o enlazar este ítem:
https://www.arca.fiocruz.br/handle/icict/20418
Tipo
ArtículoDerechos de autor
Acceso abierto
Colecciones
Metadatos
Mostrar el registro completo del ítem
IGG SUBCLASSES RESPONSIBLE FOR IMMUNE CLEARANCE IN MICE INFECTED WITH TRYPANOSOMA CRUZI
Autor
Afiliación
Institulo Butantan. Immunology Research and Training Center. São Paulo, SP, Brazil
Institulo Butantan. Immunology Research and Training Center. São Paulo, SP, Brazil
Federal University of Bahia. Health Sciences Institute. Laboratory of Immunology and Molecular Biology. Bioaggression Department. Salvador, BA, Brazil
Federal University of Bahia. Health Sciences Institute. Laboratory of Immunology and Molecular Biology. Bioaggression Department. Salvador, BA, Brazil
Institulo Butantan. Immunology Research and Training Center. São Paulo, SP, Brazil
Federal University of Bahia. Health Sciences Institute. Laboratory of Immunology and Molecular Biology. Bioaggression Department. Salvador, BA, Brazil
Federal University of Bahia. Health Sciences Institute. Laboratory of Immunology and Molecular Biology. Bioaggression Department. Salvador, BA, Brazil
Resumen en ingles
To examine the role of different immunoglobulin subclasses in the immune clearance of
Trypanosoma cruzi, mice containing bloodstream trypomastigotes were injeeted intravenously with
immune serum, IgG-depleted serum, or with thelgGI or IgG2 fraelionsand the rateof removal of the
parasites from circulation was determined. Using IgG concentrations similar to those found in the
immune serum, the rate of clearanee mediated by IgG2 was six-fold higher than that obtained with
IgGI. This differenee did not appear to be due to differences in antibody specificity, as Western
blotting showed that each isotype recognized a similar set of antigens extracted from the parasite.
However, the T. cruzi specific antibody content of the IgG2 was approximately five-fold higher than
IgG I - When the dose of IgG was adjusted to equalize the antibody content, the clearance ability of the
IgGI and IgG2 was very similar. It is concluded Ihat the two subclasses have a similar clearance
ability.
Compartir