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STEM CELL RIBONOMICS: RNA-BINDING PROTEINS AND GENE NETWORKS IN STEM CELL DIFFERENTIATION
RNA-Binding Proteins
Stem Cells
Gene Regulatory Networks
Cell Differentiation
Células Madre
Redes Reguladoras de Genes
Diferenciación Celular
Afiliación
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Resumen en ingles
Stem cells are undifferentiated cells with the ability to self-renew and the potential to differentiate into all body cell types. Stem cells follow a developmental genetic program and are able to respond to alterations in the environment through various signaling pathways. The mechanisms that control these processes involve the activation of transcription followed by a series of post-transcriptional events. These post-transcriptional steps are mediated by the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs creating a regulatory gene network. Characterizing these RNA-protein networks is essential to understanding the regulatory mechanisms underlying the control of stem cell fate. Ribonomics is the combination of classical biochemical purification protocols with the high-through put identification of transcripts applied to the functional characterization of RNA-protein complexes. Here, we describe the different approaches that can be used in a ribonomic approach and how they have contributed to understanding the function of several RBPs with central roles in stem cell biology.
Palabras clave en portugues
Diferenciação CelularPalabras clave en ingles
RibonomicsRNA-Binding Proteins
Stem Cells
Gene Regulatory Networks
Cell Differentiation
Palabras clave
Proteínas de Unión al ARNCélulas Madre
Redes Reguladoras de Genes
Diferenciación Celular
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