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2028-01-01
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MCSM-AB: A WEB SERVER FOR PREDICTING ANTIBODY-ANTIGEN AFFINITY CHANGES UPON MUTATION WITH GRAPH-BASED SIGNATURES
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/University of Cambridge. Department of Biochemistry. Cambridge, UK/University of Melbourne. Department of Biochemistry.Victoria, Australia
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/University of Cambridge. Department of Biochemistry. Cambridge, UK/University of Melbourne. Department of Biochemistry.Victoria, Australia
Abstract
Computational methods have traditionally struggled to predict the effect of mutations in antibody-antigen complexes on binding affinity. This has limited their usefulness during antibody engineering and development, and their ability to predict biologically relevant escape mutations. Here we present mCSM-AB, a user-friendly web server for accurately predicting antibody-antigen affinity changes upon mutation which relies on graph-based signatures. We show that mCSM-AB performs better than comparable methods that have been previously used for antibody engineering. mCSM-AB web server is available at http://structure.bioc.cam.ac.uk/mcsm_ab.
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