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DIRECT DETECTION OF ENTEROVIRUS GENOME IN CELL-CULTURE NEGATIVE CEREBROSPINAL FLUID FROM ASEPTIC MENINGITIS IN BRAZIL
Meningite Asséptica
Reação em Cadeia da Polimerase em Tempo Real
Líquido Cefalorraquidiano
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Abstract
Enteroviruses are the main etiologic agents involved in outbreaks and sporadic cases of aseptic meningitis. Viral
isolation in permissive cells is a traditional method for diagnosis, but it is time consuming and may be unsuccessful
due to low viral titers and because some enteroviruses do not replicate in routine cell cultures. Therefore, molecular
techniques are increasingly being used for detection of these agents. In the present work, 267 cerebrospinal fluid
(CSF) samples from aseptic meningitis and meningoencephalitis cases, received between 2008 and 2009, were tested
by RT-PCR. These clinical samples were previously negative for virus isolation in cell culture. Enterovirus genome
was detected in 59 CSF samples (22.1%) and identification was confirmed by partial nucleotide sequencing. Some
demographics of the patients included in the study were analyzed. Direct detection of enterovirus genome from CSF
is appropriate to increase sensitivity in aseptic meningitis and meningoencephalitis cases.
Keywords in Portuguese
EnterovírusMeningite Asséptica
Reação em Cadeia da Polimerase em Tempo Real
Líquido Cefalorraquidiano
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