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ArtículoDerechos de autor
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Fecha del embargo
2030-01-01
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- IOC - Artigos de Periódicos [12500]
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LOW-LEVEL LASER THERAPY (LLLT) ACTS AS CAMP-ELEVATING AGENT IN ACUTE RESPIRATORY DISTRESS SYNDROME
Fator de Necrose Tumoral
AMP Cíclico
Terapia com Luz de Baixa Intensidade
Inflamação pulmonar aguda
Alveolar macrophages
Cyclic AMP
TNF
Low-level laser therapy
Autor
Afiliación
Instituto de Pesquisa e Desenvolvimento-Urbanova, São José dos Campos, SPp, Brasil.
Departamento de Engenharia de Biosistemas. São João del Rei, MG, Brasil.
Unicastelo. Instituto de Engenharia Biomédica. São José dos Campos, SP, Brasil.
Centro Universitário Nove de Julho. Departamento de Ciências da reabilitação. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.
Departamento de Engenharia de Biosistemas. São João del Rei, MG, Brasil.
Unicastelo. Instituto de Engenharia Biomédica. São José dos Campos, SP, Brasil.
Centro Universitário Nove de Julho. Departamento de Ciências da reabilitação. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal de São Paulo. Departamento de Ciência e Tecnologia. São José dos Campos, SP, Brasil.
Resumen en ingles
The aim of this work was to investigate if the low-level laser therapy (LLLT) on acute lung inflammation (ALI) induced by lipopolysaccharide (LPS) is linked to tumor necrosis factor (TNF) in alveolar macrophages (AM) from bronchoalveolar lavage fluid (BALF) of mice. LLLT has been reported to actuate positively for relieving the late and early symptoms of airway and lung inflammation. It is not known if the increased TNF mRNA expression and dysfunction of cAMP generation observed in ALI can be influenced by LLLT. For in vivo studies, Balb/c mice (n = 5 for group) received LPS inhalation or TNF intra nasal instillation and 3 h after LPS or TNF-α, leukocytes in BALF were analyzed. LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 660 nm and a dose of 4.5 J/cm(2). The mice were irradiated 15 min after ALI induction. In vitro AM from mice were cultured for analyses of TNF mRNA expression and protein and adenosine3':5'-cyclic monophosphate (cAMP) levels. One hour after LPS, the TNF and cAMP levels in AM were measured by ELISA. RT-PCR was used to measure TNF mRNA in AM. The LLLT was inefficient in potentiating the rolipram effect in presence of a TNF synthesis inhibitor. LLLT attenuated the neutrophil influx and TNF in BALF. In AM, the laser increased the cAMP and reduced the TNF-α mRNA. LLLT increases indirectly the cAMP in AM by a TNF-dependent mechanism.
Palabras clave en portugues
Macrófagos AlveolaresFator de Necrose Tumoral
AMP Cíclico
Terapia com Luz de Baixa Intensidade
Inflamação pulmonar aguda
Palabras clave en ingles
Acute lung inflammationAlveolar macrophages
Cyclic AMP
TNF
Low-level laser therapy
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