Author | Oliveira, Nathalia Silva | |
Author | Andrade, Cecilia V. | |
Author | Grinsztejn, Beatriz | |
Author | Friedman, Ruth K. | |
Author | Cunha, Cynthia B. | |
Author | Veloso, Valdiléa G. | |
Author | Coutinho, Jose R. | |
Author | Chicarino-Oliveira, Janice M. | |
Author | Ferreira, Dennis de C. | |
Author | Pereira, Luiza O. R. | |
Author | Amaro-Filho, Sergio M. | |
Author | Moreira, Miguel A. | |
Author | Nuovo, Gerard J. | |
Author | Nicol, Alcina F. | |
Access date | 2017-11-13T14:43:39Z | |
Available date | 2017-11-13T14:43:39Z | |
Document date | 2016 | |
Citation | OLIVEIRA, Nathalia Silva etal. In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients. International Journal of Clinical and Experimental Pathology, v. 9, n. 2, p. 1520-1532, 2016. | pt_BR |
ISSN | 1936-2625 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23127 | |
Language | eng | pt_BR |
Publisher | e-Century Publishing | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | HPV/HIV-1 | pt_BR |
Subject in Portuguese | Neoplasia Intraepitelial Anal | pt_BR |
Subject in Portuguese | Resposta Imune | pt_BR |
Subject in Portuguese | Genótipo | pt_BR |
Title | In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients | pt_BR |
Type | Article | pt_BR |
Abstract | Human papillomavirus (HPV) is the main etiologic agent of lower genital tract cancers. The natural history
of HPV infection and the immune response to HIV/HPV co-infection, particularly in the anal mucosa, is poorly understood.
The aim was evaluate the in situ immune response in anorectal biopsies from HIV-infected patients. A total of
114 biopsies were analyzed by Tissue Micro-Array: 15 were from HIV-negative individuals with normal squamous epithelium
and 99 from HIV-positive individuals (21 normal squamous epithelium, 39 with anal intra-epithelial lesion
grade 1 and 39 with anal intra-epithelial lesion grade 2/3). PCR and sequencing were used to identify HPV DNA.
Staining for CD4, CD8, Foxp3+, T-bet and IL-10 were analyzed via immunohistochemistry. HIV-positive patients with
AIN 2/3 showed a lower number of CD4+ cells (< 50 cells/mm3) compared to HIV negative subjects (P = 0.01). HIV
infected individuals showed a higher expression of FoxP3+ and IL-10 that correlated with the severity of the lesion
(P = 0.002). A positive coefficient correlation was found between FoxP3+ and IL-10 (r = 0.34; P = 0.027). HPV DNA
was detected in 93.4% (101/107) of the samples and the most common types were HPV 16 (26.9%), followed by
HPV 6 (15.7%), HPV 59 (13%) and HPV 18 (10.2%). Our results showed a strong association between the increased
T-reg cells and IL-10 expression in HIV-positive patients with AIN 2/3. HPV 16 was the most prevalent type. Our study
suggests that the immune regulatory in situ profile may favor HPV persistence in HIV-positive individuals. Further in
situ studies should be done in order to elucidate the development of anal cancer in HPV/HIV co-infected individuals. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Estácio de Sá. Departamento de Endodontia. Programa de pós graduação em odontologia. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Nacional do Cancer. Divisão de Genética. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Comprehensive Cancer Center, Ohio State University, Columbus, Ohio, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HPV/HIV-1 | pt_BR |
Subject | Anal Intraepithelial Neoplasia | pt_BR |
Subject | Immune Response | pt_BR |
Subject | Genotype | pt_BR |
DeCS | Papillomaviridae | pt_BR |
DeCS | HIV-1 | pt_BR |