| Author | Caruso, Marjolly B. | |
| Author | Trugilho, Monique R. O. | |
| Author | Higa, Luiza M. | |
| Author | Ferreira, André S. Teixeira | |
| Author | Perales, Jonas | |
| Author | Poian, Andrea T. Da | |
| Author | Zingali, Russolina B. | |
| Access date | 2017-12-07T15:16:40Z | |
| Available date | 2017-12-07T15:16:40Z | |
| Document date | 2017 | |
| Citation | CARUSO, Marjorlly B. et al. Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus. Journal of Proteomics, v.151, p.106-113, July 2017. | pt_BR |
| ISSN | 1874-3919 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23546 | |
| Language | eng | pt_BR |
| Publisher | 1876-7737 | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Vírus da Dengue | pt_BR |
| Subject in Portuguese | Hepatócitos | pt_BR |
| Subject in Portuguese | Proteoma | pt_BR |
| Subject in Portuguese | Proteínas de baixa massa molecular | pt_BR |
| Subject in Portuguese | Predição de enzimas proteolíticas | pt_BR |
| Title | Proteomic analysis of the secretome of HepG2 cells indicates differential proteolytic processing after infection with dengue virus | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.jprot.2016.07.011 | |
| Abstract | Secretome analysis can be described as a subset of proteomics studies consisting in the analysis of the molecules secreted by cells or tissues. Dengue virus (DENV) infection can lead to a broad spectrum of clinical manifestations, with the severe forms of the disease characterized by hemostasis abnormalities and liver injury. The hepatocytes are a relevant site of viral replication and a major source of plasma proteins. Until now, we had limited information on the small molecules secreted by hepatic cells after infection by DENV. In the present study, we analysed a fraction of the secretome of mock- and DENV-infected hepatic cells (HepG2 cells) containing molecules with <10kDa, using different proteomic approaches. We identified 175 proteins, with 57 detected only in the samples from mock-infected cells, 59 only in samples from DENV-infected cells, and 59 in both conditions. Most of the peptides identified were derived from proteins larger than 10kDa, suggesting a proteolytic processing of the secreted molecules. Using in silico analysis, we predicted consistent differences between the proteolytic processing occurring in mock and DENV-infected samples, raising, for the first time, the hypothesis that differential proteolysis of secreted molecules would be involved in the pathogenesis of dengue. | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Estrutural. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Dengue virus | pt_BR |
| Subject | Hepatic cells | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Low molecular mass proteins | pt_BR |
| Subject | Prediction of proteolytic enzymes | pt_BR |
| Subject | Secretome | pt_BR |
| Embargo date | 2030-01-01 | |
