Author | Pereira, Mariana M. C. | |
Author | Malvezzi, Amaranta M. | |
Author | Nascimento, Larissa M. | |
Author | Lima, Tamara D. C. da Costa | |
Author | Alves, Viviane S. | |
Author | Palma, Mariana L. | |
Author | Freire, Eden R. | |
Author | Moura, Danielle M. N. | |
Author | Reis, Christian R. S. | |
Author | Melo Neto, Osvaldo P. de | |
Access date | 2017-12-12T13:59:10Z | |
Available date | 2017-12-12T13:59:10Z | |
Document date | 2013 | |
Citation | PEREIRA, M. M. C. et al. The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture. Molecular and Biochemical Parasitology, v. 190, n. 2, p. 82–86, ago. 2013. | pt_BR |
ISSN | 1872-9428 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23622 | |
Sponsorship | O financiamento pessoal aos membros da equipe foi fornecido pelas agências brasileiras de financiamento CNPq , FACEPE e CAPES . O trabalho de laboratório foi parcialmente financiado com subsídios da FACEPE ( APQ-0557-2.02 / 10 ) e os programas PAPES IV e V da FIOCRUZ. A assistência à cultura de parasitas foi fornecida por B. Lima. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Síntese proteíca | pt_BR |
Subject in Portuguese | Iniciação | pt_BR |
Subject in Portuguese | Regulamento | pt_BR |
Subject in Portuguese | Fosforilação | pt_BR |
Title | The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.molbiopara.2013.06.008 | |
Abstract | The eukaryotic eIF4F complex, the cap binding complex, functions during translation initiation through interactions mediated by its three subunits (eIF4E, eIF4G and eIF4A), other initiation factors and the ribosome. In trypanosomatids, various eIF4E and eIF4G homologues were identified, with two eIF4F-like complexes confirmed (EIF4E4/EIF4G3/EIF4AI and EIF4E3/EIF4G4/EIF4AI). Here, the expression pattern of these complexes was investigated during Leishmania amazonensis and Trypanosoma brucei growth. The two sets of eIF4E and eIF4G homologues were found represented by phosphorylated isoforms with multiple phosphorylation events targeting the two eIF4E homologues. Expression of these multiple isoforms was differentially affected by inhibitors of mRNA synthesis/processing and translation. Phosphorylated EIF4E4 was consistently associated with early/active growth phases in both organisms studied. In T. brucei phosphorylation of both EIF4E3 and 4, overexpressed as HA-tagged fusions, was partially mapped to their N-terminuses. Our results indicate that phosphorylation is associated with a further layer of complexity in translation initiation in trypanosomatids. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Subject | Protein synthesis | pt_BR |
Subject | Initiation | pt_BR |
Subject | Regulation | pt_BR |
Subject | Phosphorylation | pt_BR |
DeCS | Fator de Iniciação 4E em Eucariotos | pt_BR |
DeCS | metabolismo | pt_BR |
DeCS | Leishmania | pt_BR |
DeCS | enzimologia | pt_BR |
DeCS | Leishmania | pt_BR |
DeCS | crescimento & desenvolvimento | pt_BR |
DeCS | Multimerização Proteica | pt_BR |
DeCS | Processamento de Proteína Pós-Traducional | pt_BR |
DeCS | Trypanosoma brucei brucei | pt_BR |
DeCS | enzimologia | pt_BR |
DeCS | Trypanosoma brucei brucei | pt_BR |
DeCS | crescimento & desenvolvimento | pt_BR |
DeCS | Fator de Iniciação 4G em Eucariotos | pt_BR |
DeCS | metabolismo | pt_BR |
DeCS | Perfilação da Expressão Gênica | pt_BR |
DeCS | Fosforilação | pt_BR |
DeCS | Isoformas de Proteínas | pt_BR |
DeCS | metabolismo | pt_BR |
DeCS | Subunidades Proteicas | pt_BR |
DeCS | metabolismo | pt_BR |