Chronic Low-Grade Inflammation in Childhood Obesity Is Associated with Decreased IL-10 Expression by Monocyte Subsets

Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Morfologia. Laboratorio de Biologia das Interacões Celulares. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Morfologia. Laboratorio de Biologia das Interacões Celulares. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Universidade Estadual de Santa Cruz. Departamento de Genetica. Ilheus, BA, Brasil/Servico de Medicina Preventiva da Unimed. Aracaju, SE, Brasil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Morfologia. Laboratorio de Biologia das Interacões Celulares. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Morfologia. Laboratorio de Biologia das Interacões Celulares. Belo Horizonte, MG, Brasil/ Instituto Nacional de Ciência e Tecnologia em Doencas Tropicais—INCT-DT
Universidade Federal de Mato Grasso. Departamento de Ciências Basicas da Saude. Faculdade de Medicina. Cuiaba, MT, Brasil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/ Instituto Nacional de Ciência e Tecnologia em Doencas Tropicais—INCT-DT
Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Morfologia. Laboratorio de Biologia das Interacões Celulares. Belo Horizonte, MG, Brasil

Abstract

Chronic low-grade inflammation is related to the development of comorbidities and poor prognosis in obesity. Monocytes are main sources of cytokines and play a pivotal role in inflammation. We evaluated monocyte frequency, phenotype and cytokine profile of monocyte subsets, to determine their association with the pathogenesis of childhood obesity. Children with obesity were evaluated for biochemical and anthropometric parameters. Monocyte subsets were characterized by flow cytometry, considering cytokine production and activation/recognition molecules. Correlation analysis between clinical parameters and immunological data delineated the monocytes contribution for low-grade inflammation. We observed a higher frequency of non-classical monocytes in the childhood obesity group (CO) than normal-weight group (NW). All subsets displayed higher TLR4 expression in CO, but their recognition and antigen presentation functions seem to be diminished due to lower expression of CD40, CD80/86 and HLA-DR. All subsets showed a lower expression of IL-10 in CO and correlation analyses showed changes in IL-10 expression profile. The lower expression of IL-10 may be decisive for the maintenance of the low-grade inflammation status in CO, especially for alterations in non-classical monocytes profile. These cells may contribute to supporting inflammation and loss of regulation in the immune response of children with obesity.

Keywords in Portuguese

obesidade infantil
resposta imune

Keywords

Monocytes
Childhood obesity
Inflammation
Cytokines
Obesity
Inflammatory diseases
Adiponectin
Immune response

Publisher

Public Library of Science

Citation

MATTOS, Rafael Teixeira de et al. Chronic Low-Grade Inflammation in Childhood Obesity Is Associated with Decreased IL-10 Expression by Monocyte Subsets. Plos One, v. 11, n. 12, art. e0168610, 2016

DOI

10.1371/journal.pone.0168610

ISSN

1932-6203

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/23647

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Open access

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