| Author | Pandolfi, Rafael | |
| Author | Almeida, Denise Ramos de | |
| Author | Pinto, Marcelo Alves | |
| Author | Kreutz, Luiz Carlos | |
| Author | Frandoloso, Rafael | |
| Access date | 2018-02-15T16:49:54Z | |
| Available date | 2018-02-15T16:49:54Z | |
| Document date | 2017 | |
| Citation | PANDOLFI, Rafael; et al. In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG antihepatitis E virus amongst blood donors in south Brazil. Plos One, v.12, n.5, e017640, 12p, May 2017. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24847 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Vírus da Hepatite E | pt_BR |
| Subject in Portuguese | Doadores de sangue | pt_BR |
| Subject in Portuguese | Sul do Brasil | pt_BR |
| Subject in Portuguese | Imunoglobulina G | pt_BR |
| Subject in Portuguese | Teste ELIZA | pt_BR |
| Subject in Portuguese | Anticorpos | pt_BR |
| Subject in Portuguese | proteína ORF2 recombinante | pt_BR |
| Title | In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0176409 | |
| Abstract | Hepatitis E Virus (HEV) is a zoonotic pathogen responsible for causing acute hepatitis in human, especially in developing countries. Diagnosis of HEV usually relies on the detection of antibodies mostly by enzyme-linked immunosorbent assay (ELISA). In the present study, we designed a new indirect ELISA (iELISA) based on a short recombinant peptide derived from the capsid protein (ORF2p) and demonstrated its potential for detecting human IgG against HEV genotype 3. The best polystyrene plate (Maxisorp®), optimal ORF2p coating antigen concentration (0,67μg/well) and primary antibody dilution (1:100) were determined. This iELISA showed a sensitivity of 91.4% and specificity of 95.9%. The comparison of our in house iELISA with a commercial assay (RecomWell, Mikrogen®) showed 94.25% of agreement and a kappa index of 0.88. The ORF2 recombinant ELISA was used to screen 780 blood donors for anti-HEV IgG and we found that 314 (40,25%) of these donors were IgG positive. This high prevalence of antibodies suggests, for the first time, that the Southern Brazil region might be endemic to Hepatitis E Virus genotype 3. | pt_BR |
| Affilliation | Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil. | pt_BR |
| Affilliation | Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil. | pt_BR |
| Affilliation | Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil. | pt_BR |
| Subject | Hepatitis E Virus | pt_BR |
| Subject | blood donors | pt_BR |
| Subject | south Brazil | pt_BR |
| Subject | ELIZA | pt_BR |
| Subject | IgG antihepatitis E virus | pt_BR |
| Subject | antibodies | pt_BR |
| Subject | recombinant ORF2 protein | pt_BR |
