Author | Carvalho, Natália Barbosa | |
Author | Prates, Fernanda Ventin de Oliveira | |
Author | Silva, Rafael de Castro da | |
Author | Dourado, Mayra Elizabeth Ferreira | |
Author | Amorim, Camila Farias | |
Author | Machado, Paulo Roberto Lima | |
Author | Pacheco, Fernanda Grendene | |
Author | Corte, Temis Weber Furlanetto | |
Author | Machado, Pablo | |
Author | Santos, Diógenes Santiago | |
Author | Carvalho, Edgar Marcelino de | |
Access date | 2018-02-21T19:27:05Z | |
Available date | 2018-02-21T19:27:05Z | |
Document date | 2017 | |
Citation | CARVALHO, N. B. et al. In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis. Journal of Immunology Research, 3062892, 2017. | pt_BR |
ISSN | 2314-8861 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/24905 | |
Sponsorship | Ministry of Health, National Council for Scientific and Technological Development (CNPq) from Brazil
(467207/2014-3), and Coordination for the Improvement of Higher Education Personnel (CAPES). | pt_BR |
Language | eng | pt_BR |
Publisher | Hindawi Publishing Corporation | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose Cutânea | pt_BR |
Subject in Portuguese | Purina-nucleósideo fosforilase | pt_BR |
Subject in Portuguese | Linfócitos | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Citometria de fluxo | pt_BR |
Subject in Portuguese | Humanos | pt_BR |
Title | In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2017/3062892 | |
Abstract | Cutaneous leishmaniasis (CL) is the most common clinical form of American tegumentary leishmaniasis caused byLeishmania(Viannia)braziliensis. CL is associated with a strong Th1 immune response. This exacerbated inflammatory response is correlated with severity of disease and delays the healing time of the ulcer. The fourth-generation immucillin derivative (DI4G), a potent inhibitor of purine nucleoside phosphorylase, has been proposed as a promising agent in the treatment of diseases associated with T cell activation. Herein, we evaluated thein vitroimmunomodulatory activity of DI4G in cells of patients presenting with CL. Peripheral blood mononuclear cells (PBMC) from CL patients were stimulated with soluble leishmania antigen (SLA), in the presence or absence of DI4G, and IFN-γ, TNF, CXCL9, and CXCL10 levels were determined by ELISA. Lymphocyte proliferation in the presence or absence of DI4G was also evaluated, using flow cytometry. DI4G was able to decrease (p< 0.05) IFN-γproduction but did not change the TNF, CXCL9, and CXCL10 levels. DI4G decreased (p< 0.05) the lymphoproliferative response mediated by CD8+T cells, but not that by CD4+T cells. DI4G is able to attenuate the exaggerated immune response in CL, exhibiting immunomodulatory activity in IFN-γproduction and in CD8+T cell proliferation. | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, Brasil | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, Brasil | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, Brasil | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Subject | Cutaneous Leishmaniasis | pt_BR |
Subject | Purine Nucleoside Phosphorylase | pt_BR |
Subject | Lymphocyte | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | Humans | pt_BR |