Author | Gardinassi, Luiz Gustavo Araujo | |
Author | Vieira, Thiago Soares de Souza | |
Author | Silva, Naila O. da | |
Author | Garcia, Gustavo Rocha | |
Author | Borges, Valéria de Matos | |
Author | Campos, Roseane Nunes de Santana | |
Author | Almeida, Roque Pacheco de | |
Author | Santos, Isabel Kiney Ferreira de Miranda | |
Author | Saraiva, Elvira Maria | |
Access date | 2018-02-23T18:34:28Z | |
Available date | 2018-02-23T18:34:28Z | |
Document date | 2017 | |
Citation | GARDINASSI, L. G. A. et al.Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis. Parasites & Vectors, v. 10, p. 285, 2017. | pt_BR |
ISSN | 1756-3305 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/24926 | |
Sponsorship | Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de
Pessoal de Nível Superior (CAPES). LGG and GRG were supported by scholarships from the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (2011/23819-0 and 2014/25856-8; 2013/00382-0, respectively). | pt_BR |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Armadilhas extracelulares de Neutrófilos | pt_BR |
Subject in Portuguese | NETosis | pt_BR |
Subject in Portuguese | Leishmania infantum | pt_BR |
Subject in Portuguese | Leishmaniose visceral | pt_BR |
Subject in Portuguese | Infecção assintomática | pt_BR |
Title | Molecular signatures of neutrophil extracellular traps in human visceral leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13071-017-2222-5 | |
Abstract | Infections with parasites of the Leishmania donovani complex result in clinical outcomes that range from asymptomatic infection to severe and fatal visceral leishmaniasis (VL). Neutrophils are major players of the immune response against Leishmania, but their contribution to distinct states of infection is unknown. Gene expression data suggest the activation of the NETosis pathway during human visceral leishmaniasis. Thus, we conducted an exploratory study to evaluate NET-related molecules in retrospective sera from VL patients, asymptomatic individuals and uninfected endemic controls. Results: We demonstrate that VL patients and asymptomatic individuals exhibit differential regulation of molecules
associated with neutrophil extracellular traps (NET). These differences were observed at the transcriptional level of
genes encoding NET-associated proteins; in quantifications of cell free DNA and metalloproteinase 9; and in enzymatic
activity of DNAse and elastase. Moreover, multivariate analysis resulted in class-specific signatures, and ROC curves
demonstrate the ability of these molecules in discriminating asymptomatic infection from uninfected controls.
Conclusion: Molecules that are associated with NETs are differentially regulated between distinct states of infection
with L. infantum, suggesting that NETs might have distinct roles depending on the clinical status of infection. Although
unlikely to be exclusive for VL, these signatures can be useful to better characterize asymptomatic infections in
endemic regions of this disease. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, Brazil | pt_BR |
Affilliation | Universidade Federal de Sergipe. Hospital Universitário. Departamento de Medicina. Aracaju, SE, Brazil | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil | pt_BR |
Subject | Neutrophil extracellular traps | pt_BR |
Subject | NETosis | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | Asymptomatic infection | pt_BR |