Author | Medeiros, R. Bambino | |
Author | Oliveira, F. O. R. | |
Author | Calvet, C. M. | |
Author | Vicente, D. | |
Author | Toma, L. | |
Author | Krieger, M. A. | |
Author | Meirelles, M. N. | |
Author | Pereira, M. C. S. | |
Access date | 2018-03-20T16:51:00Z | |
Available date | 2018-03-20T16:51:00Z | |
Document date | 2011 | |
Citation | MEDEIROS, R. Bambino; et al. Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion. Parasitology, v.138, p.593-601, 2011. | pt_BR |
ISSN | 0031-1820 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25435 | |
Language | eng | pt_BR |
Publisher | Cambridge University Press | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Miócitos Cardíacos | pt_BR |
Subject in Portuguese | processo de reconhecimento | pt_BR |
Subject in Portuguese | Heparitina Sulfato | pt_BR |
Title | Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion | pt_BR |
Type | Article | pt_BR |
DOI | 10.1017/S0031182010001678 | |
Abstract | Cell surface glycosaminoglycans (GAGs) play an important role in the attachment and invasion process of a variety of intracellular pathogens. We have previously demonstrated that heparan sulfate proteoglycans (HSPG) mediate the invasion of trypomastigote forms of Trypanosoma cruzi in cardiomyocytes. Herein, we analysed whether GAGs are also implicated in amastigote invasion. Competition assays with soluble GAGs revealed that treatment of T. cruzi amastigotes with heparin and heparan sulfate leads to a reduction in the infection ratio, achieving 82% and 65% inhibition of invasion, respectively. Other sulfated GAGs, such as chondroitin sulfate, dermatan sulfate and keratan sulfate, had no effect on the invasion process. In addition, a significant decrease in infection occurred after interaction of amastigotes with GAG-deficient Chinese Hamster Ovary (CHO) cells, decreasing from 20% and 28% in wild-type CHO cells to 5% and 9% in the mutant cells after 2 h and 4 h of infection, respectively. These findings suggest that amastigote invasion also involves host cell surface heparan sulfate proteoglycans. The knowledge of the mechanism triggered by heparan sulfate-binding T. cruzi proteins may provide new potential candidates for Chagas disease therapy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | cardiomyocytes | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | amastigotes | pt_BR |
Subject | heparan sulfate | pt_BR |
Subject | recognition process | pt_BR |
e-ISSN | 1469-8161 | |
Embargo date | 2030-01-01 | |