Author | Melo, C. M. L. de | |
Author | Melo, H. | |
Author | Correia, M. T. S. | |
Author | Coelho, L. C. B. B. | |
Author | Silva, M. B. da | |
Author | Pereira, V. R. A. | |
Access date | 2018-04-23T15:31:58Z | |
Available date | 2018-04-23T15:31:58Z | |
Document date | 2011 | |
Citation | DE MELO, C. M. L. et al. Mitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice. Scandinavian Journal of Immunology, v. 73, n. 2, p. 112–121, fev. 2011. | pt_BR |
ISSN | 1365-3083 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/26032 | |
Sponsorship | Este trabalho foi financiado pelo Conselho Nacional de Pesquisa (CNPq), pela Coordenação de Estudos Avançados em Educação Superior (CAPES) e pelo Centro de Pesquisas Aggeu Magalhães, da Fundação Oswaldo Cruz, em Recife (CPqAM / FIOCRUZ). | pt_BR |
Language | eng | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Cramoll 1,4 | pt_BR |
Subject in Portuguese | Cratylia mollis | pt_BR |
Subject in Portuguese | resposta imunoestimuladora | pt_BR |
Subject in Portuguese | Lectinas | pt_BR |
Title | Mitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/j.1365-3083.2010.02490.x | |
Abstract | Cramoll 1,4 is a lectin with specific glucose/mannose binding, which is extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine expression activity and anti-inflammatory profile of this lectin. The aim of this study was to evaluate the immunostimulatory response, in vitro, of splenocytes in mice previously inoculated, in vivo, with C. mollis (Cramoll 1,4) and Canavalia ensiformis (Con A) lectins. Results demonstrated higher proliferation indexes induced by Cramoll 1,4 than Con A lectin in relation to all experimental groups. Cramoll 1,4 and Con A also induced high levels of IL-2, IL-6, IFN-γ and nitric oxide production. Moreover, Cramoll 1,4 did not induce apoptosis and stimulated a significant number of cells in the S phase of the cell cycle. Results showed that Cramoll 1,4 lectin induces proliferative response and suggested that this lectin can be used as a mitogenic agent in immunostimulatory assays. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Análises Estatísticas. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Centro de Ciências Biológicas. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Departamento de Biofísica. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Subject | Cramoll 1,4 | pt_BR |
Subject | Cratylia mollis | pt_BR |
Subject | immunostimulatory response | pt_BR |
Subject | lectin | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Proliferação celular | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Interferon gama / biossíntese | pt_BR |
DeCS | Interferon gama / imunologia | pt_BR |
DeCS | Interleucina-2 / biossíntese | pt_BR |
DeCS | Interleucina-2 / imunologia | pt_BR |
DeCS | Interleucina-6 / biossíntese | pt_BR |
DeCS | Interleucina-6 / imunologia | pt_BR |
DeCS | Linfócitos / citologia | pt_BR |
DeCS | Linfócitos / imunologia | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Ratos | pt_BR |
DeCS | Mitose | pt_BR |
DeCS | Óxido Nítrico / biossíntese | pt_BR |
DeCS | Lectinas vegetais / imunologia | pt_BR |
DeCS | Baço / citologia | pt_BR |
DeCS | Baço / imunologia | pt_BR |
Embargo date | 2050-01-01 | |