Author | Ferreira, Lígia Maria | |
Author | Romão, Tatiany Patrícia | |
Author | Melo Neto, Osvaldo Pompílio de | |
Author | Silva Filha, Maria Helena Neves Lobo | |
Access date | 2018-04-26T13:59:55Z | |
Available date | 2018-04-26T13:59:55Z | |
Document date | 2010 | |
Citation | FERREIRA, L. M. et al. The orthologue to the Cpm1/Cqm1 receptor in Aedes aegypti is expressed as a midgut GPI-anchored α-glucosidase, which does not bind to the insecticidal binary toxin. Insect Biochemistry and Molecular Biology, v. 40, n. 8, p. 604–610, ago. 2010. | pt_BR |
ISSN | 1879-0240 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/26130 | |
Sponsorship | Este estudo foi apoiado pelas agências de fomento brasileiras Conselho Nacional de Pesquisa (CNPq Brasil, bolsa 471911 / 2006-2) e Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, outorga APQ 0427-2.13 / 08). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Toxina bin | pt_BR |
Subject in Portuguese | Bacillus sphaericus | pt_BR |
Subject in Portuguese | Biolarvicidas | pt_BR |
Subject in Portuguese | Receptor | pt_BR |
Subject in Portuguese | Ligação de toxinas | pt_BR |
Subject in Portuguese | Glicosilação | pt_BR |
Title | The orthologue to the Cpm1/Cqm1 receptor in Aedes aegypti is expressed as a midgut GPI-anchored α-glucosidase, which does not bind to the insecticidal binary toxin | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.ibmb.2010.05.007 | |
Abstract | Aedes aegypti larvae are refractory to the insecticidal binary (Bin) toxin from Bacillus sphaericus, which is not able to bind to its target tissue in the larval midgut. In contrast, Culex pipiens larvae are highly susceptible to that toxin, which targets its midgut brush border membranes (BBMF) through the binding of the BinB subunit to specific receptors, the Cpm1/Cqm1 membrane-bound α-glucosidases. The identification of an Ae. aegypti gene encoding a Cpm1/Cqm1 orthologue, here named Aam1, led to the major goal of this study which was to investigate its expression. The aam1 transcript was found in larvae and adults from Ae. aegypti and a ≈73-kDa protein was recognized by an anti-Cqm1 antibody in midgut BBMF. The Aam1 protein displayed α-glucosidase activity and localized to the midgut epithelium, bound through a GPI anchor, similarly to Cpm1/Cqm1. However, no binding of native Aam1 was observed to the recombinant BinB subunit. Treatment of both proteins with endoglycosidase led to changes in the molecular weight of Aam1, but not Cqm1, implying that the former was glycosylated. The findings from this work rule out lack of receptors in larval stages, or its expression as soluble proteins, as a reason for Ae. aegypti refractoriness to Bin toxin. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Subject | Bin toxin | pt_BR |
Subject | Bacillus sphaericus | pt_BR |
Subject | Biolarvicides | pt_BR |
Subject | Receptor | pt_BR |
Subject | Toxin binding | pt_BR |
Subject | Glycosylation | pt_BR |
DeCS | Aedes / enzimologia | pt_BR |
DeCS | Aedes / genética | pt_BR |
DeCS | Aedes / crescimento e desenvolvimento | pt_BR |
DeCS | Aedes / metabolismo | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Toxinas bacterianas / metabolismo | pt_BR |
DeCS | Sistema Digestivo / enzimologia | pt_BR |
DeCS | Sistema Digestivo / metabolismo | pt_BR |
DeCS | Expressão genetica | pt_BR |
DeCS | Glicosilfosfatidilinositóis / metabolismo | pt_BR |
DeCS | Proteínas de Insetos / genética | pt_BR |
DeCS | Proteínas de Insetos / metabolismo | pt_BR |
DeCS | Inseticidas / metabolismo | pt_BR |
DeCS | Larva / enzimologia | pt_BR |
DeCS | Larva / genética | pt_BR |
DeCS | Larva / metabolismo | pt_BR |
DeCS | Dados de Sequencia Molecular | pt_BR |
DeCS | Ligação proteica | pt_BR |
DeCS | alfa-glucosidases / genética | pt_BR |
DeCS | alfa-glucosidases / metabolismo | pt_BR |
Embargo date | 2050-01-01 | |