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https://www.arca.fiocruz.br/handle/icict/2653
PARTICIPATION OF CYTOKINES IN THE NECROTIC-INFLAMMATORY LESIONS IN THE HEART AND SKELETAL MUSCLES OF CALOMYS CALLOSUS INFECTED WITH TRYPANOSOMA CRUZI
Calomys callosus
Cytokines
Tumor necrosis factor-alpha
Gamma interferon
Tumor growth factor-beta
Citocinas
Músculo Esquelético
Trypanosoma cruzi
Miocárdio
Fator Transformador de Crescimento beta
Fator de Necrose Tumoral alfa
Interferon gama
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract
Calomys callosus, a sylvatic reservoir of Trypanosoma cruzi, when infected with the Colombian strain (Biodeme
Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal
muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor
necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis
of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were
inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls.
After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle
were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized
skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence
of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ
search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected
C. callosus was successful. The cytokines TNF-α, IFN-γ , and TGF-β were detected in the cytoplasm of macrophages
and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance
by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation
of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and
in the regression of fibrotic-inflammatory lesions observed in C. callosus.
Keywords
Trypanosoma cruziCalomys callosus
Cytokines
Tumor necrosis factor-alpha
Gamma interferon
Tumor growth factor-beta
DeCS
Doença de ChagasCitocinas
Músculo Esquelético
Trypanosoma cruzi
Miocárdio
Fator Transformador de Crescimento beta
Fator de Necrose Tumoral alfa
Interferon gama
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