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https://www.arca.fiocruz.br/handle/icict/27974
DIAGNOSTIC PERFORMANCE OF COMMERCIAL IGM AND IGG ENZYME-LINKED IMMUNOASSAYS (ELISAS) FOR DIAGNOSIS OF ZIKA VIRUS INFECTION
Sensibilidade e especificidade
Ensaio imunoenzimático
Imunoglobulina M
Imunoglobulina G
Sensitivity and specificity
Enzyme-linked immunosorbent assay
Immunoglobulin M
Immunoglobulin G
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Medicina Tropical CONICET. Puerto Iguazu, Misiones, Argentina
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
University of Texas Medical Branch. Galveston, TX, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Emory University. Atlanta, GA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Medicina Tropical CONICET. Puerto Iguazu, Misiones, Argentina
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
University of Texas Medical Branch. Galveston, TX, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Emory University. Atlanta, GA, USA
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Abstract
Serologic detection of Zika virus (ZIKV) infections is challenging because of antigenic similarities among flaviviruses. Objective: To evaluate the sensitivity and specificity of commercial ZIKV IgM and IgG enzyme-linked immunoassay
(ELISA) kits.
Methods: We used sera from febrile patients with RT-PCR-confirmed ZIKV infection to determine sensitivity and
sera from RT-PCR-confirmed dengue cases and blood donors, both of which were collected before ZIKV epidemics
in Brazil (2009–2011 and 2013, respectively) to determine specificity.
Results: The ZIKV IgM-ELISA positivity among RT-PCR ZIKV confirmed cases was 0.0% (0/14) and 12.5% (1/8) for
acute- and convalescent-phase sera, respectively, while its specificity was 100.0% (58/58) and 98.3% (58/59) for
acute- and convalescent-phase sera of dengue patients, and 100.0% (23/23) for blood donors. The ZIKV IgG-ELISA
sensitivity was 100.0% (6/6) on convalescent-phase sera from RT-PCR confirmed ZIKV patients, while its specificity
was 27.3% (15/55) on convalescent-phase sera from dengue patients and 45.0% (9/20) on blood donors’ sera. The
ZIKV IgG-ELISA specificity among dengue confirmed cases was much greater among patients with primary dengue
(92.3%; 12/13), compared to secondary dengue (7.1%; 3/42).
Conclusions: In a setting of endemic dengue transmission, the ZIKV IgM-ELISA had high specificity, but poor
sensitivity. In contrast, the ZIKV IgG-ELISA showed low specificity, particularly for patients previously exposed to
dengue infections. This suggests that this ZIKV IgM-ELISA is not useful in confirming a diagnosis of ZIKV infection in
suspected patients, whereas the IgG-ELISA is more suitable for ZIKV diagnosis among travelers, who reside in areas
free of flavivirus transmission, rather than for serosurveys in dengue-endemic areas.
Keywords in Portuguese
Zika virusSensibilidade e especificidade
Ensaio imunoenzimático
Imunoglobulina M
Imunoglobulina G
Keywords
Zika virusSensitivity and specificity
Enzyme-linked immunosorbent assay
Immunoglobulin M
Immunoglobulin G
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