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Fecha del embargo
2030-01-01
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- IOC - Artigos de Periódicos [12500]
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LOCALIZATION OF HEMATOPOIETIC CELLS IN THE BULLFROG (LITHOBATES CATESBEIANUS)
Histoquímica
Imunohistoquímica
Anfíbios
Lithobates catesbeianus [Anura]
Histochemistry
Imunohistochemistry
Amphibian
Lithobates catesbeianus [Anura]
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biológicas. Laboratório e Histologia Comparativa e Animal. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biológicas. Laboratório e Histologia Comparativa e Animal. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.
Resumen en ingles
Amphibians represent the first phylogenetic group to possess hematopoietic bone marrow. However, adult amphibian hematopoiesis has only been described in a few species and with conflicting data. Bone marrow, kidney, spleen, liver, gut, stomach, lung, tegument, and heart were therefore collected from adult Lithobates catesbeianus and investigated by light microscopy and immunohistochemical methods under confocal laser microscopy. Our study demonstrated active hematopoiesis in the bone marrow of vertebrae, femur, and fingers and in the kidney, but no hematopoietic activity inside other organs including the spleen and liver. Blood cells were identified as a heterogeneous cell population constituted by heterophils, basophils, eosinophils, monocytes, erythrocytic cells, lymphocytes, and their precursors. Cellular islets of the thrombocytic lineage occurred near sinusoids of the bone marrow. Antibodies against CD34, CD117, stem cell antigen, erythropoietin receptor, and the receptor for granulocyte colony-stimulating factor identified some cell populations, and some circulating immature cells were seen in the bloodstream. Thus, on the basis of these phylogenetic features, we propose that L. catesbeianus can be used as an important model for hematopoietic studies, since this anuran exhibits hematopoiesis characteristics both of lower vertebrates (renal hematopoiesis) and of higher vertebrates (bone marrow hematopoiesis).
Palabras clave en portugues
HematopoeseHistoquímica
Imunohistoquímica
Anfíbios
Lithobates catesbeianus [Anura]
Palabras clave en ingles
HematopoiesisHistochemistry
Imunohistochemistry
Amphibian
Lithobates catesbeianus [Anura]
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