Author | Tan, Paul Thiamjoo | |
Author | Heiny, A. T. | |
Author | Miotto, Olivo | |
Author | Salmon, Jerome | |
Author | Marques, Ernesto T. A. | |
Author | Lemonnier, Francois | |
Author | August, J. Thomas | |
Access date | 2018-08-31T13:57:40Z | |
Available date | 2018-08-31T13:57:40Z | |
Document date | 2010 | |
Citation | TAN, P. T. et al. Conservation and diversity of influenza A H1N1 HLA-restricted T cell epitope candidates for epitope-based vaccines. PloS One, v. 5, n. 1, p. e8754, 18 jan. 2010. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28496 | |
Sponsorship | Instituto Nacional de Alergia e Doenças Infecciosas, Institutos Nacionais de Saúde, Departamento de Saúde e Serviços Humanos, EUA sob Grant No. 5 U19 AI56541 e Contrato No. HHSN2662-00400085C. | pt_BR |
Language | eng | pt_BR |
Publisher | Derya Unutmaz, New York University, United States of America | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células T | pt_BR |
Subject in Portuguese | Vírus da influenza A | pt_BR |
Subject in Portuguese | H1N1 | pt_BR |
Subject in Portuguese | Banco de Dados de Seqüência | pt_BR |
Title | Conservation and diversity of influenza A H1N1 HLA-restricted T cell epitope candidates for epitope-based vaccines | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0008754 | |
Abstract | BACKGROUND:
The immune-related evolution of influenza viruses is exceedingly complex and current vaccines against influenza must be reformulated for each influenza season because of the high degree of antigenic drift among circulating influenza strains. Delay in vaccine production is a serious problem in responding to a pandemic situation, such as that of the current H1N1 strain. Immune escape is generally attributed to reduced antibody recognition of the viral hemagglutinin and neuraminidase proteins whose rate of mutation is much greater than that of the internal non-structural proteins. As a possible alternative, vaccines directed at T cell epitope domains of internal influenza proteins, that are less susceptible to antigenic variation, have been investigated.
METHODOLOGY/PRINCIPAL FINDINGS:
HLA transgenic mouse strains expressing HLA class I A*0201, A*2402, and B*0702, and class II DRB1*1501, DRB1*0301 and DRB1*0401 were immunized with 196 influenza H1N1 peptides that contained residues of highly conserved proteome sequences of the human H1N1, H3N2, H1N2, H5N1, and avian influenza A strains. Fifty-four (54) peptides that elicited 63 HLA-restricted peptide-specific T cell epitope responses were identified by IFN-gamma ELISpot assay. The 54 peptides were compared to the 2007-2009 human H1N1 sequences for selection of sequences in the design of a new candidate H1N1 vaccine, specifically targeted to highly-conserved HLA-restricted T cell epitopes.
CONCLUSIONS/SIGNIFICANCE:
Seventeen (17) T cell epitopes in PB1, PB2, and M1 were selected as vaccine targets based on sequence conservation over the past 30 years, high functional avidity, non-identity to human peptides, clustered localization, and promiscuity to multiple HLA alleles. These candidate vaccine antigen sequences may be applicable to any avian or human influenza A virus. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
Affilliation | National University of Singapore. Yong Loo Lin School of Medicine. Department of Biochemistry. Singapore. | pt_BR |
Affilliation | Oxford University. Centre for Genomics and Global Health. Oxford, United Kingdom / Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Research Unit. Bangkok, Thailand. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
Affilliation | University of Pittsburgh. Center for Vaccine Research. Pittsburgh, Pennsylvania, United States of America / University of Pittsburgh. Department of Infectious Diseases and Microbiology. Pittsburgh, Pennsylvania, United States of America / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Unité Immunité Cellulaire Antivirale. Paris, France. | pt_BR |
Affilliation | Johns Hopkins University. School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, Maryland, United States of America. | pt_BR |
Subject | T cells | pt_BR |
Subject | Influenza A virus | pt_BR |
Subject | H1N1 | pt_BR |
Subject | Sequence databases | pt_BR |
DeCS | Sequência de Aminoácidos | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Epítopos / química | pt_BR |
DeCS | Epitopos / imunologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Vírus da Influenza A Subtipo H1N1 / imunologia | pt_BR |
DeCS | Vacinas contra Influenza / imunologia | pt_BR |
DeCS | Ratos | pt_BR |
DeCS | Ratos transgênicos | pt_BR |
DeCS | Dados de Sequencia Molecular | pt_BR |
DeCS | Linfócitos T / imunologia | pt_BR |