Author | Cruz, Wagner Baetas da | |
Author | Alves, Lucineia | |
Author | Pessolani, Maria Cristina V. | |
Author | Barbosa, Helene S. | |
Author | Régnier-Vigouroux, Anne | |
Author | Corte-Real, Suzana | |
Author | Cavalcante, Leny A. | |
Access date | 2018-09-20T14:10:06Z | |
Available date | 2018-09-20T14:10:06Z | |
Document date | 2009 | |
Citation | CRUZ, Wagner Baetas da; et al. Schwann cells express the macrophage mannose receptor and MHC class II. Do they have a role in antigen presentation?. Journal of the Peripheral Nervous System, v.14, p.84–92, 2009. | pt_BR |
ISSN | 1085-9489 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28936 | |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | receptor de reconhecimento de padrões | pt_BR |
Subject in Portuguese | lesão nervosa | pt_BR |
Subject in Portuguese | imunidade inata | pt_BR |
Subject in Portuguese | proteína manosilada | pt_BR |
Subject in Portuguese | Glia | pt_BR |
Title | Schwann cells express the macrophage mannose receptor and MHC class II. Do they have a role in antigen presentation? | pt_BR |
Type | Article | pt_BR |
Abstract | The mannose receptor (MR) is a transmembrane glycoprotein, postulated to
be a link between innate and adaptive immunity. MR is expressed in several cell types but
no information is available on that for Schwann cells (SC).We show that rodent SC in primary
cultures take up the MR ligand mannosyl/bovine serum albumin-fluorescein isothiocyanate
(man/BSA-FITC) in a highly specific manner and bind an antibody against the C-terminus
of the murine macrophage MR (anti-cMR). After incubation with man/BSA-FITC, flow
cytometry demonstrates 90% positive SC, a dose-dependent increase in tagged cellular
components and near total inhibition of the neoglycoprotein uptake by D-mannose or by the
mannosylated protein horseradish peroxidase (HRP). Western blot for MR shows that SC
share a unique protein of about 180 kDa with peritoneal resident macrophages. Treatment
of cultured SC with interferon-γ (IFN-γ ) or dexamethasone (DM) followed by the addition of
man/BSA-FITC and analysis by flow cytometry shows down- or upregulation, respectively,
of man/BSA-FITC uptake. Our results show that SC express the MR in a prospectively
functional state and suggest an antigen-presenting function of SC, compatible with a role
in infectious/inflammatory states of the peripheral nervous system. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Programa de Terapia Celular e Bioengenharia. Laboratório de Neurobiologia do Desenvolvimento. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-Estrutura e Biologia Celular. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-Estrutura e Biologia Celular. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | 4INSERM U701, DKFZ, INF 242. Heidelberg, Germany. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-Estrutura e Biologia Celular. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Programa de Terapia Celular e Bioengenharia. Laboratório de Neurobiologia do Desenvolvimento. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | glia | pt_BR |
Subject | pattern recognition receptor | pt_BR |
Subject | innate immunity | pt_BR |
Subject | mannosylated protein | pt_BR |
Subject | nerve injury | pt_BR |
Embargo date | 2030-01-01 | |