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VIRULENCE AND CELLULAR INTERACTIONS OF BURKHOLDERIA MULTIVORANS IN CHRONIC GRANULOMATOUS DISEASE
Virulência
Interações celulares
Burkholderia multivorans
Author
Affilliation
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA / NIAID and Microbiology Service. Clinical Center. Bethesda, Maryland, USA.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
National Institutes of Health. Division of Veterinary Research. Bethesda, Maryland, USA.
National Institutes of Health. Division of Veterinary Research. Bethesda, Maryland, USA.
National Institutes of Health. Biological Imaging Section. Bethesda, Maryland, USA.
United States Army Medical Research Institute of Infectious Diseases. Bacteriology Division. Fort Detrick, Maryland, USA.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
University of Virginia Health System. Department of Microbiology. Charlottesville, Virignia, USA.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
National Institutes of Health. Division of Veterinary Research. Bethesda, Maryland, USA.
National Institutes of Health. Division of Veterinary Research. Bethesda, Maryland, USA.
National Institutes of Health. Biological Imaging Section. Bethesda, Maryland, USA.
United States Army Medical Research Institute of Infectious Diseases. Bacteriology Division. Fort Detrick, Maryland, USA.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
University of Virginia Health System. Department of Microbiology. Charlottesville, Virignia, USA.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
National Institutes of Health. Laboratory of Clinical Infectious Diseases. Bethesda, Maryland, USA
Abstract
Chronic granulomatous disease (CGD) patients are susceptible to life-threatening infections by the
Burkholderia cepacia complex. We used leukocytes from CGD and healthy donors and compared cell
association, invasion, and cytokine induction by Burkholderia multivorans strains. A CGD isolate, CGD1,
showed higher cell association than that of an environmental isolate, Env1, which correlated with cell
entry. All B. multivorans strains associated significantly more with cells from CGD patients than with those
from healthy donors. Similar findings were observed with another CGD pathogen, Serratia marcescens, but
not with Escherichia coli. In a mouse model of CGD, strain CGD1 was virulent while Env1 was avirulent.
B. multivorans organisms were found in the spleens of CGD1-infected mice at levels that were 1,000 times
higher than those found in Env1-infected mice, which was coincident with higher levels of the proinflammatory
cytokine interleukin-1 . Taken together, these results may shed light on the unique susceptibility
of CGD patients to specific pathogens.
Keywords in Portuguese
Doença granulomatosa crônicaVirulência
Interações celulares
Burkholderia multivorans
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