Author | Moraes, C. S. | |
Author | Martins, K. Aguiar | |
Author | Costa, S. G. | |
Author | Bates, P. A. | |
Author | Dillon, R. J. | |
Author | Genta, F. A. | |
Access date | 2018-10-02T15:34:28Z | |
Available date | 2018-10-02T15:34:28Z | |
Document date | 2018 | |
Citation | MORAES, C. S. et al. Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection. BioMed Research International, Volume 2018, Article ID2472508, 10p, 2018. | pt_BR |
ISSN | 2314-613 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29239 | |
Language | eng | pt_BR |
Publisher | Hindawi Publishing Corporation | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Lutzomyia longipalpis | pt_BR |
Subject in Portuguese | fêmea | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | segunda refeição de sangue | pt_BR |
Subject in Portuguese | oviposição | pt_BR |
Title | Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1155/2018/2472508 | |
Abstract | Lutzomyia longipalpis is the main vector of visceral leishmaniasis (VL) in America. Physiological and molecular mechanisms of Leishmania infection in sand flies have been studied during the first gonotrophic cycle. There are few studies about these interactions during the second gonotrophic cycle mainly because of the difficulties maintaining sand flies through sequential feeds. Here we standardized conditions to perform the second blood feed efficiently, and our results show that oviposition is an essential factor for the success of multiple feeds. We evaluated the impact of the second blood meal on longevity, protein digestion, trypsin activity, and Leishmania mexicana development within L. longipalpis gut. Mortality of blood-fed females increases after second blood meal as compared to sugar-fed females. Trypsin activity was lower during the second gonotrophic cycle. However, no difference in protein intake was observed between blood meals. There was no difference in the population size of Leishmania in the gut after both blood meals. In this work, we presented an optimized protocol for obtaining sufficient numbers of sand fly females fed on a second blood meal, and we described some physiological and parasitological aspects of the second gonotrophic cycle which might influence the vectorial competence of sand flies. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK. | pt_BR |
Affilliation | Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK. | pt_BR |
Affilliation | Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK. | pt_BR |
Affilliation | Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Cidade Universitária. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Cidade Universitária. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Lutzomyia longipalpis | pt_BR |
Subject | Second Blood Meal | pt_BR |
Subject | female | pt_BR |
Subject | oviposition | pt_BR |
e-ISSN | 2314-6141 | |