Author | Queiroz, Conceição | |
Author | Barral Netto, Manoel | |
Author | Bacchi, Carlos Eduardo | |
Access date | 2018-10-04T16:52:33Z | |
Available date | 2018-10-04T16:52:33Z | |
Document date | 2001 | |
Citation | QUEIROZ, C.; BARRAL NETTO, M.; BACCHI, C. E. Characterizing subpopulations of neoplastic cells in serous effusions. The role of immunocytochemistry. Acta Cytologica, v. 45, n.1, p. 18-22, 2001. | pt_BR |
ISSN | 0001-5547 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29353 | |
Description | Barral Netto, Manoel. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Language | eng | pt_BR |
Publisher | Karger Publishers | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células | pt_BR |
Subject in Portuguese | Neoplasias | pt_BR |
Subject in Portuguese | Imunocitoquímica | pt_BR |
Subject in Portuguese | Efusões serosas | pt_BR |
Title | Characterizing subpopulations of neoplastic cells in serous effusions. The role of immunocytochemistry | pt_BR |
Type | Article | pt_BR |
DOI | 10.1159/000327182 | |
Abstract | To analyze the role of immunochemistry in serous effusions. STUDY DESIGN: We analyzed cell blocks of 18 pleural
and 18 peritoneal effusions
diagnosed as malignant (18),
benign (14) and suspicious
(4). They were immunostained
by the avidin-biotin
complex method with a panel
of four monoclonal antibodies—
CEA, Ber-EP4, LeuM1
(CD15) and p53—and, for
lectins (Ulex europaeus)
UEA-l, ConA and ConBr.
RESULTS: Seventeen of the
18 cases of adenocarcinoma were positive for CEA
(95%), 12 (66.6%) for Ber-EP4, 11 (61%) for CD15 and
11 (61%) for p53. Twelve of the 18 (66.6%) were positive
for UEA-1, CEA, Ber-EP4 and CD15. UEA-1 did not
react with mesothelial cells. p53 Gave a positive reaction
in only one case, reactive mesothelial cells. ConA and
ConBr reacted indiscriminately with benign and malignant
cells; thus, it was not useful in distinguishing between
these cells.
CONCLUSION: In this context no antibody used alone
is reliable for corroborating a diagnosis, but the selective
use of a small panel of three markers (CEA, Ber-EP4 and
LeuM1) can be very useful in solving diagnostic difficulties
in the cytodiagnosis of
serous effusions. | pt_BR |
Affilliation | Federal University of Bahia. Department of Gynecology, Obstetrics and Human Reproduction. Salvador, BA, Brazil | pt_BR |
Affilliation | Federal University of Bahia. Department of Anatomic Pathology and Immunology. Salvador, BA, Brazil | pt_BR |
Affilliation | Universidade Estadual Paulista. Department of Pathology. São Paulo, SP, Brazil | pt_BR |
Subject | Cells | pt_BR |
Subject | Neoplasms | pt_BR |
Subject | Immunocytochemistry | pt_BR |
Subject | Serous effusions | pt_BR |