Author | Silva, Alexandre dos Santos da | |
Author | Vasconcelos, Gentil Arthur Lins Bentes Mendonça de | |
Author | Kappel, Livia Abud | |
Author | Pinto, Marcelo Alves | |
Author | Paula, Vanessa Salete de | |
Access date | 2018-11-06T12:24:59Z | |
Available date | 2018-11-06T12:24:59Z | |
Document date | 2012 | |
Citation | SILVA, Alexandre dos Santos da; et al. An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y. Mem Inst Oswaldo Cruz, v.107, n.7, p.960-963, Nov. 2012. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29884 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | IgY | pt_BR |
Subject in Portuguese | Hepatite A | pt_BR |
Subject in Portuguese | ELISA | pt_BR |
Title | An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y | pt_BR |
Type | Article | pt_BR |
Abstract | The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody levels using anti-HAV IgY as the capture and conjugated immunoglobulins. For this purpose, anti-HAV IgY was conjugated to horseradish peroxidase (HRP) and the optimal dilution of HRP-conjugated antibodies was evaluated to establish the competitive immuneenzymatic assay. The results obtained from our “in-house” assay were plotted on a receiver operator curve, which showed a sensitivity of 95% and a specificity of 98.8%, demonstrating that a competitive anti-HAV IgY immunoenzymatic assay developed “in house” could be used as an alternative to commercial assays that utilise IgG. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Centro Universitário Serra dos Órgãos. Escola de Medicina Veterinária. Teresópolis, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Centro Universitário Serra dos Órgãos. Escola de Medicina Veterinária. Teresópolis, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | IgY | pt_BR |
Subject | hepatitis A | pt_BR |
Subject | ELISA | pt_BR |
e-ISSN | 1678-8060 | |