Author | Silva, Roberta Peres da | |
Author | Martins, Sharon de Toledo | |
Author | Rizzo, Juliana | |
Author | Reis, Flavia Coelho Garcia dos | |
Author | Joffe, Luna S. | |
Author | Vainstein, Marilene Henning | |
Author | Kmetzsch, Lívia | |
Author | Oliveira, Débora L. | |
Author | Puccia, Rosana | |
Author | Goldenberg, Samuel | |
Author | Rodrigues, Marcio Lourenço | |
Author | Alves, Lysangela Ronalte | |
Access date | 2018-11-13T16:37:36Z | |
Available date | 2018-11-13T16:37:36Z | |
Document date | 2018 | |
Citation | SILVA, Roberta Peres da et al. Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans. Genes, v. 9, n. 8, p. 1-17, 2018. | pt_BR |
ISSN | 2073-4425 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30015 | |
Language | por | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Title | Golgi Reassembly and Stacking Protein (GRASP) participates in vesicle-mediated RNA export in cryptococcus neoformans | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/genes9080400 | |
Abstract | Golgi reassembly and stacking protein (GRASP) is required for polysaccharide secretion and virulence in Cryptococcus neoformans. In fungal species, extracellular vesicles (EVs) participate in the export of polysaccharides, proteins and RNA. In the present work, we investigated if EV-mediated RNA export is functionally connected with GRASP in C. neoformans using a graspΔ mutant. Since GRASP-mediated unconventional secretion involves autophagosome formation in yeast, we included the atg7Δ mutant with defective autophagic mechanisms in our analysis. All fungal strains exported EVs but deletion of GRASP or ATG7 profoundly affected vesicular dimensions. The mRNA content of the graspΔ EVs differed substantially from that of the other two strains. The transcripts associated to the endoplasmic reticulum were highly abundant transcripts in graspΔ EVs. Among non-coding RNAs (ncRNAs), tRNA fragments were the most abundant in both mutant EVs but graspΔ EVs alone concentrated 22 exclusive sequences. In general, our results showed that the EV RNA content from atg7Δ and WT were more related than the RNA content of graspΔ, suggesting that GRASP, but not the autophagy regulator Atg7, is involved in the EV export of RNA. This is a previously unknown function for a key regulator of unconventional secretion in eukaryotic cells. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / School of Life Sciences. University of Nottingham. Nottingham, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | Extracellular Vesicles | pt_BR |
Subject | Golgi Apparatus | pt_BR |
Subject | Autophagy-Related Protein 7 | pt_BR |
Subject | Secretory Pathway | pt_BR |
Subject in Spanish | ARN | pt_BR |
Subject in Spanish | Aparato de Golgi | pt_BR |
Subject in Spanish | Proteína 7 Relacionada con la Autofagia | pt_BR |
Subject in Spanish | Vías Secretoras | pt_BR |
DeCS | Cryptococcus neoformans | pt_BR |
DeCS | RNA | pt_BR |
DeCS | Vesículas Extracelulares | pt_BR |
DeCS | Complexo de Golgi | pt_BR |
DeCS | Proteína 7 Relacionada à Autofagia | pt_BR |
DeCS | Via Secretória | pt_BR |
e-ISSN | 2073-4425 | |