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ArtículoDerechos de autor
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Fecha del embargo
2030-01-01
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- IOC - Artigos de Periódicos [12512]
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PRIMARY SKELETAL MUSCLE CELLS TRIGGER SPONTANEOUS TOXOPLASMA GONDII TACHYZOITE-TO-BRADYZOITE CONVERSION AT HIGHER RATES THAN FIBROBLASTS
Persistência do parasita
Células musculares esqueléticas
Conversão espontânea
taquizoítos
bradizitos
fibroblastos
Skeletal muscle cell
Stage conversion
Bradyzoite
Parasite persistence
tachyzoite
fibroblasts
Autor
Afiliación
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Georg-August-University. Institute for Medical Microbiology. Göttingen, Germany.
Resumen en ingles
Toxoplasma gondii is one of the most common eukaryotic parasites and an important opportunistic pathogen of humans. The interconversion from proliferative tachyzoites into quiescent encysted bradyzoites plays pivotal roles in the lifelong persistence of T. gondii in its host and the pathogenesis of toxoplasmosis. Stage conversion and persistence in skeletal muscle tissue may be particularly important for the food-borne transmission of T. gondii to humans via raw or undercooked meat products. Here, we have followed the transition of tachyzoites to bradyzoites after infection of skeletal muscle cells (SkMC). Primary murine myoblasts were differentiated to multinucleated syncytial myotubes that displayed regular contractions in vitro and expressed myogenic markers Myf5 and MyoD. Tachyzoites of T. gondii invaded SkMC within 4h of infection and started to replicate within 24h of infection. Remarkably, intracellular tachyzoites readily differentiated to bradyzoites in SkMC without the need of exogenous stress factors. Double immunofluorescence labelling revealed significantly higher percentages of bradyzoite-containing vacuoles in SkMC than in murine fibroblasts at 24h until 6 days after infection. Furthermore, transcript levels of bradyzoite-specific ENO1 but not tachyzoite-specific ENO2 strongly increased in T. gondii-infected SkMC until 6 days of infection. These findings indicate that the commitment of T. gondii to differentiate to bradyzoites in SkMC does not require exogenous stress factors but could be rather regulated by cell-type specific factors.
Palabras clave en portugues
Toxoplasma gondiiPersistência do parasita
Células musculares esqueléticas
Conversão espontânea
taquizoítos
bradizitos
fibroblastos
Palabras clave en ingles
Toxoplasma gondiiSkeletal muscle cell
Stage conversion
Bradyzoite
Parasite persistence
tachyzoite
fibroblasts
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