Mostrar el registro sencillo del ítem
Autor | Graham, Robert I. | |
Autor | Zahner, Viviane | |
Autor | Lucarotti, Christopher J. | |
Fecha de acceso | 2019-02-28T13:00:41Z | |
Fecha de disponibilización | 2019-02-28T13:00:41Z | |
Fecha de publicación | 2008 | |
Referencia | GRAHAM, Robert I.; ZAHNER, Viviane; LUCAROTTI, Christopher J. An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta). Can. J. Microbiol., v.54, p.758–768, 2008. | pt_BR |
ISSN | 0008-4166 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31916 | |
Idioma | eng | pt_BR |
Editor | NRC Research Press | pt_BR |
Derechos de autor | restricted access | pt_BR |
Palabras clave en Portugués | Bactéria | pt_BR |
Palabras clave en Portugués | Wolbachia. | pt_BR |
Palabras clave en Portugués | endosimbionte | pt_BR |
Palabras clave en Portugués | Hymenoptera | pt_BR |
Palabras clave en Portugués | moscas | pt_BR |
Título | An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta) | pt_BR |
Tipo del documento | Article | pt_BR |
DOI | 10.1139/W08-067 | |
Resumen en Inglés | Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR– DGGE and PCR–RFLP products revealed a dominance of a- and g-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata). | pt_BR |
Afiliación | University of New Brunswick. Faculty of Forestry and Environmental Management. Population Ecology Group. Fredericton, Canada. | pt_BR |
Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Afiliación | University of New Brunswick. Faculty of Forestry and Environmental Management. Population Ecology Group. Fredericton, Canada / Canadian Forest Service. Atlantic Forestry Centre. Natural Resources Canada. Fredericton, Canada. | pt_BR |
xmlui.metadata.dc.description.abstractfr | Six espe`ces de mouches a` scie (Hyme´nopte`res : Symphytes) appartenant a` quatre familles taxonomiques (Agridae, Diprionidae, Pamphiliidae et Tenthredinidae) ont e´te´ recueillies dans diffe´rentes localite´s a` travers le Canada et ont e´te´ examine´es quant a` la pre´sence de microbiotes associe´s. L’ADN total a e´te´ extrait des insectes individuellement et une re´action en chaine par polyme´rase (PCR) a e´te´ utilise´e pour amplifier la re´gion conserve´e du ge`ne de l’ARNr 16S des microbiotes. Des e´lectrophore`ses sur gel en gradient de´naturant (DGGE) et des analyses de polymorphisme de longueur des fragments de restriction (RFLP) ont e´te´ re´alise´es pour se´parer les clones bacte´riens associe´s aux insectes hoˆtes. Le se´- quenc¸age des produits obtenus en PCR–DGGE et en PCR–RFLP a re´ve´le´ une dominance des prote´obacte´ries a et g, la plupart des se´quences montrant un haut degre´ de similarite´ avec les bacte´ries identifie´es pre´ce´demment chez d’autres insectes et chez d’autres e´chantillons environnementaux. De plus, une souche de l’endosymbiote bacte´rien Wolbachia et un bacte´riophage de Wolbachia ont e´te´ identifie´s du Tenthre`de du sorbier (Pristiphora geniculata). | pt_BR |
Palavras clave en Inglês | Bacteria | pt_BR |
Palavras clave en Inglês | endosymbiont | pt_BR |
Palavras clave en Inglês | Hymenoptera | pt_BR |
Palavras clave en Inglês | sawflies | pt_BR |
Palavras clave en Inglês | Wolbachia | pt_BR |
Palavras clave en Francês | Bactérie | pt_BR |
Palavras clave en Francês | Wolbachia. | pt_BR |
Palavras clave en Francês | Endosymbiote | pt_BR |
Palavras clave en Francês | Hyménoptère | pt_BR |
Palavras clave en Francês | Mouches a` scie | pt_BR |
e-ISSN | 1480-3275 | |
Fecha de embargo | 2030-01-01 |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
IOC - Artigos de Periódicos [12500]