Author | Fampa, P. | |
Author | Lisboa, C. V. | |
Author | Jansen, Ana Maria | |
Author | Santos, A. L. S. | |
Author | Ramirez, M. I. | |
Access date | 2019-03-14T13:40:52Z | |
Available date | 2019-03-14T13:40:52Z | |
Document date | 2008 | |
Citation | FAMPA, P. et al. Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups. Parasitology, v. 135, p. 1093–1100, 2008. | pt_BR |
ISSN | 0031-1820 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32080 | |
Language | eng | pt_BR |
Publisher | Cambridge University Press | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Interação parasita-hospedeiro | pt_BR |
Subject in Portuguese | Fatores de Virulência | pt_BR |
Subject in Portuguese | Proteases | pt_BR |
Subject in Portuguese | Genótipos TCI e TCII | pt_BR |
Title | Protease expression analysis in recently field-isolated strains of Trypanosoma cruzi: a heterogeneous profile of cysteine protease activities between TC I and TC II major phylogenetic groups | pt_BR |
Type | Article | pt_BR |
DOI | 10.1017/S0031182008004587 | |
Abstract | Protease expression among TCI and TCII field isolates was analysed. Gelatin-containing gels revealed hydrolysis bands
with molecular masses ranging from 45 to 66 kDa. The general protease expression profile showed that TCII isolates
presented higher heterogeneity compared to TCI. By utilizing protease inhibitors, we showed that all active proteases at
acid pH are cysteine-proteases and all proteases active at alkaline pH are metalloproteases. However, the expression of
cruzipain, the T. cruzi major cysteine-protease, did not reproduce a heterogeneous TCII cysteine zymogram profile.
Dendogram analyses based on presence/absence matrices of proteases and cruzipain bands showed a TCI separation from
the TCII group with 50–60% similarity. We suggest that the observed cysteine protease diversification contributes to
differential host infection between TCI and II genotypes. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebotomíneos. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | TCI and TCII genotypes | pt_BR |
Subject | Proteases | pt_BR |
Subject | Cruzipain | pt_BR |
Subject | Host-parasite interaction | pt_BR |
Subject | Virulence | pt_BR |
e-ISSN | 1469-8161 | |
Embargo date | 2022-01-01 | |