Author | Tomlinson, Stephen | |
Author | Pontes-de-Carvalho, Lain Carlos | |
Author | Vandekerckhove, Filip | |
Author | Nussenzweig, Victor | |
Access date | 2019-03-29T13:27:36Z | |
Available date | 2019-03-29T13:27:36Z | |
Document date | 1992 | |
Citation | TOMLINSON, S. et al. Resialylation of sialidase-treated sheep and human erythrocytes by Trypanosoma cruzi trans-sialidase: restoration of complement resistance of desialylated sheep erythrocytes. Glycobiology, v. 2, n. 6, p. 549-551, 1992. | pt_BR |
ISSN | 0959-6658 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32292 | |
Description | Pontes-de-Carvalho, Lain Carlos. Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
“Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | N1H grant AI32966, by the John D. and Catherine T.MacArthur Foundation,
by the United Nations Development Program/World Health Organization
Special Program for Research and Training in Tropical Diseases, and by the
Rockefeller Foundation. F.V. is a research assistant of the Belgian National
Fund for Scientific Research and L.P.C. is a senior investigator of the Oswaldo
Cruz Foundation and is a recipient of a grant from the RHAE program,
Secretary of Science and Technology, Brazil. | pt_BR |
Language | eng | pt_BR |
Publisher | Oxford University Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Eritrócitos | pt_BR |
Subject in Portuguese | Complemento | pt_BR |
Subject in Portuguese | Ácido sialidase | pt_BR |
Subject in Portuguese | Acido sialico | pt_BR |
Subject in Portuguese | Trans-sialidase | pt_BR |
Title | Resialylation of sialidase-treated sheep and human erythrocytes by Trypanosoma cruzi trans-sialidase: restoration of complement resistance of desialylated sheep erythrocytes | pt_BR |
Type | Article | pt_BR |
Abstract | Trypanosoma cruzi trans-sialidase (TS) is a recently
described enzyme whkh transfers a(2-3)-linked sialic acid
from host-derived sialylated glycoconjugates to parasite
surface molecules [Schenkman et al. (1991) Cell, 65, 1117].
We report here on the ability of TS to transfer sialic acid
from donor sialyl-a(2-3)lactose to sialidase-treated sheep
and human erythrocytes. Up to ~ 50% resialylation of both
desialylated red cells could be attained. Resialylation of
desialylated sheep erythrocytes restores their resistance to
lysis by human complement. This ascribes a possible
biological role for T.cruzi TS and demonstrates directly that
sialic acid is solely responsible for preventing alternative
pathway activation of human complement by sheep
erythrocytes. | pt_BR |
Affilliation | New York University Medical Center. Department of Pathology. New York, NY, USA. | pt_BR |
Affilliation | New York University Medical Center. Department of Pathology. New York, NY, USA. | pt_BR |
Affilliation | New York University Medical Center. Department of Pathology. New York, NY, USA. | pt_BR |
Affilliation | New York University Medical Center. Department of Pathology. New York, NY, USA. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Erythrocytes | pt_BR |
Subject | Complement | pt_BR |
Subject | Acid sialidase | pt_BR |
Subject | Sialic acid | pt_BR |
Subject | Trans-sialidase | pt_BR |