Author | Medeiros, Fernanda Silva | |
Author | Martins, Albert Eduardo Silva | |
Author | Gomes, Renan Garcia | |
Author | Oliveira, Sávio Augusto Vieira de | |
Author | Welkovic, Stefan | |
Author | Maruza, Magda | |
Author | Menezes, Maria Luiza Bezerra | |
Author | Ximenes, Ricardo Arraes de Alencar | |
Author | Diniz, George Tadeu Nunes | |
Author | Donadi, Eduardo Antônio | |
Author | Lucena-Silva, Norma | |
Access date | 2019-04-08T12:43:47Z | |
Available date | 2019-04-08T12:43:47Z | |
Document date | 2018 | |
Citation | MEDEIROS, F. S. et al. Variation Sites at the HLA-G 3’ Untranslated Region Confer Differential Susceptibility to HIV/HPV Co-Infection and Aneuploidy in Cervical Cell. PloS One, v. 13, n. 10, p. 1-14, 2018. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32404 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Variation sites at the HLA-G 3' untranslated region confer differential susceptibility to HIV/HPV co-infection and aneuploidy in cervical cell | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0204679 | |
Abstract | Post-transcriptional regulatory elements associated with transcript degradation or transcript instability have been described at the 3' untranslated region (3'UTR) of the HLA-G gene. Considering that HPV infection and aneuploidy, which causes gene instability, are associated with cervical cell malignancy, as well as the fact that HIV infection and HLA-G may modulate the immune response, the present study aimed to compare the frequencies of HLA-G 3'UTR polymorphic sites (14-base pair insertion/deletion, +3142C/G, and +3187A/G) between 226 HIV+ women co-infected (n = 82) or not with HPV (n = 144) and 138 healthy women. We also evaluated the relationship between those HLA-G 3'UTR variants and aneuploidy in cervical cells. HPV types and HLA-G polymorphisms were determined by PCR and sequencing of cervical samples DNA. Aneuploidy in cervical cell was measured by flow cytometry. The HLA-G 3'UTR 14-bp ins/del was not associated with either HIV nor HIV/HPV co-infection. The +3142G allele (p = 0.049) and +3142GG genotype (p = 0.047) were overrepresented in all HIV-infected women. On the other hand, the +3187G allele (p = 0.028) and the +3187GG genotype (p = 0.026) predominated among healthy women. The +3142G (p = 0.023) and +3187A (p = 0.003) alleles were associated with predisposition to HIV infection, irrespective of the presence or not of HIV/HPV co-infection. The diplotype formed by the combination of the +3142CX (CC or CG) and +3187AA genotype conferred the highest risk for aneuploidy in cervical cell induced by HPV. The HLA-G 3'UTR +3142 and +3187 variants conferred distinct susceptibility to HIV infection and aneuploidy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunogenética. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunogenética. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunogenética. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunogenética. Recife, PE, Brasil. | pt_BR |
Affilliation | University of Pernambuco. Integrated Health Centre Amaury de Medeiros. Recife, PE, Brazil. | pt_BR |
Affilliation | Health Secretariat of Pernambuco. Hospital Correia Picanço. Recife, PE, Brazil. | pt_BR |
Affilliation | University of Pernambuco. Maternal and Child Department of the Faculty of Medical Sciences. Recife, PE, Brazil. | pt_BR |
Affilliation | Federal University of Pernambuco. Department of Tropical Medicine. Recife, PE, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Pública. Laboratório de Métodos Computacionais. Recife, PE, Brazil. | pt_BR |
Affilliation | University of São Paulo. School of Medicine of Ribeirão Preto. Department of Medicine. Ribeirão Preto, SP, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Laboratório de Imunogenética. Recife, PE, Brasil / IMIP Hospital. Pediatric Oncology Service. Recife, PE, Brazil. | pt_BR |
Subject | HLA-G 3' | pt_BR |
Subject | HIV/HPV | pt_BR |
Subject | Aneuploidy in cervical cell | pt_BR |
DeCS | 3 'Regiões não traduzidas | pt_BR |
DeCS | Adulto | pt_BR |
DeCS | Aneuploidia | pt_BR |
DeCS | Brasil | pt_BR |
DeCS | Estudos de caso-controle | pt_BR |
DeCS | Cervix Uteri / imunologia | pt_BR |
DeCS | Uteri do colo do útero / virologia | pt_BR |
DeCS | Coinfecção / genética | pt_BR |
DeCS | Coinfecção / imunologia | pt_BR |
DeCS | Fêmea | pt_BR |
DeCS | Predisposição Genética para Doença | pt_BR |
DeCS | Infecções por HIV / genética | pt_BR |
DeCS | Infecções por HIV / imunologia | pt_BR |
DeCS | Antígenos HLA-G / genética | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Infecções por papilomavírus / genética | pt_BR |
DeCS | Infecções por papilomavírus / imunologia | pt_BR |
DeCS | Polimorfismo Genético | pt_BR |