Autor | Dopico, Eva | |
Autor | Del-Rei, Rodrigo Pimenta | |
Autor | Espinoza, Bertha | |
Autor | Ubillos, Itziar | |
Autor | Zanchin, Nilson Ivo Tonin | |
Autor | Sulleiro, Elena | |
Autor | Moure, Zaira | |
Autor | Celedon, Paola Alejandra Fiorani | |
Autor | Souza, Wayner Vieira | |
Autor | Silva, Edimilson Domingos da | |
Autor | Gomes, Yara Miranda | |
Autor | Santos, Fred Luciano Neves | |
Data de acesso | 2019-04-11T18:23:25Z | |
Data de disponibilização | 2019-04-11T18:23:25Z | |
Data do publicação | 2019 | |
Citação | DOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019. | pt_BR |
ISSN | 1471-2334 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32455 | |
Fomento | This research project was funded by a Gonçalo Moniz Institute, Oswaldo
Cruz Foundation (Fiocruz/BA). | pt_BR |
Idioma | eng | pt_BR |
Editor | BMC | pt_BR |
Direito Autoral | open access | pt_BR |
Palavras-chave | Doença de Chagas | pt_BR |
Palavras-chave | Trypanosoma cruzi | pt_BR |
Palavras-chave | Antígenos Quiméricos | pt_BR |
Palavras-chave | Imunoensaio | pt_BR |
Palavras-chave | Precisão | pt_BR |
Título | Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting | pt_BR |
Tipo do documento | Article | pt_BR |
DOI | 10.1186/s12879-019-3872-z | |
Resumo em Inglês | Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Methods: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive
and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from
pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies.
Results: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively,
demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive
samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from
99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and
99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was
similar to that for negative Chagas disease individuals.
Conclusions: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA
and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in nonendemic
settings. | pt_BR |
Afiliação | Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain. | pt_BR |
Afiliação | Faculty of Technology and Sciences of Bahia. Salvador, Bahia, Brazil. | pt_BR |
Afiliação | Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas. Departamento de Inmunología. Ciudad de México, Mexico. | pt_BR |
Afiliação | Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Afiliação | Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain. | pt_BR |
Afiliação | Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain. | pt_BR |
Afiliação | Institute of Paraná. Molecular Biology. Curitiba, PR, Brazil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. | pt_BR |
Afiliação | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Palavras-chave em inglês | Chagas disease | pt_BR |
Palavras-chave em inglês | Trypanosoma cruzi | pt_BR |
Palavras-chave em inglês | Chimeric antigens | pt_BR |
Palavras-chave em inglês | Immunoassay | pt_BR |
Palavras-chave em inglês | Accuracy | pt_BR |
DeCS | Adulto | pt_BR |
DeCS | Anticorpos Antiprotozoários | pt_BR |
DeCS | Imunologia | pt_BR |
DeCS | Antígenos de Protozoários | pt_BR |
DeCS | Genética | pt_BR |
DeCS | Doença de Chagas | pt_BR |
DeCS | Diagnóstico | pt_BR |
DeCS | Parasitologia | pt_BR |
DeCS | Reações Cruzadas | pt_BR |
DeCS | Emigrantes e Imigrantes | pt_BR |
DeCS | Estatística & dados numéricos | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Gravidez | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |
DeCS | Espanha | pt_BR |
DeCS | Toxoplasma | pt_BR |
DeCS | Trypanosoma cruzi | pt_BR |